REV PROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 AFFECTS THE STABILITY AND TRANSPORT OF THE VIRAL MESSENGER-RNA
REV PROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 AFFECTS THE STABILITY AND TRANSPORT OF THE VIRAL MESSENGER-RNA
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DOI：
10.1073/pnas.86.5.1495
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发表时间：
1989-03-01
影响因子：
11.1
通讯作者：
PAVLAKIS, GN
中科院分区：
文献类型：
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作者：
FELBER, BK;HADZOPOULOUCLADARAS, M;PAVLAKIS, GN
REV(trs/art)是人类免疫缺陷病毒1型(HIV-1)必需的调节蛋白。REV通过HIV-1的env区域中的一个顺式作用元件，称为rev反应元件，增加产生Gag和env的mRNAs的水平。我们的结果表明，REV增加了未剪接病毒mRNA的稳定性，而它不影响不包含REV反应元件的多重剪接病毒mRNAs的稳定性。对产生不能剪接的mRNA的突变前病毒结构的研究表明，rev对稳定性的影响不依赖于剪接。我们的实验还表明，REV促进了含有REV反应元件的病毒mRNA从细胞核到细胞质的运输。提出的REV的功能与免疫荧光显示的其核定位一致。REV对病毒mRNA水平的选择性作用表明，REV是一种反馈调节模型，导致病毒表达的稳定状态。
rev (trs/art) is an essential human immunodeficiency virus type 1 (HIV-1) regulatory protein. rev increases the levels of the gag- and env-producing mRNAs via a cis-acting element in the env region of HIV-1, named rev-responsive elements. Our results show that rev increases the stability of the unspliced viral mRNA, while it does not affect the stability of the multiply spliced viral mRNAs that do not contain the rev-responsive element. The study of mutated proviral constructs producing mRNA that cannot be spliced revealed that the effect of rev on stability is independent of splicing. Our experiments also indicate that rev promotes the transport of the viral mRNA containing the rev-responsive element from the nucleus to the cytoplasm. The proposed functions of rev are consistent with its nuclear localization as shown by immunofluorescence. The selective effects of rev on the levels of the viral mRNA suggest a model for feedback regulation by rev leading to a steady state of viral expression.