Durable Alteration of the Colonic Microbiota by the Administration of Donor Fecal Flora
Durable Alteration of the Colonic Microbiota by the Administration of Donor Fecal Flora
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DOI：
10.1097/mcg.0b013e3181e5d06b
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发表时间：
2010-09-01
影响因子：
2.9
通讯作者：
Wettstein, Antony
中科院分区：
文献类型：
--
作者：
Grehan, Martin J.;Borody, Thomas Julius;Wettstein, Antony
目的：确定粪便细菌治疗是否会对菌群相关疾病患者的结肠微生物群产生持久有益的改变。背景：早期的研究表明，结肠微生物群在许多情况下都有影响。将健康个体的粪便悬浮液施用于患病个体（粪便细菌疗法）可以治愈艰难梭菌感染和潜在的其他疾病。口服益生菌在这种情况下不起作用，但没有研究确定粪便细菌治疗是否会延长植入时间。研究：收集10例接受粪便细菌治疗的患者的粪便样本。在输注健康捐赠者的粪便之前，患者完成了抗生素治疗和肠道清洗。采用分子方法，从预处理到首次输注后24周，跟踪患者粪便样本中的细菌数量，并与初始输注的供体粪便悬浮液进行比较。结果：术后4周、8周和24周，患者粪便样本中的细菌主要由来自健康供体样本的细菌组成。比较4周、8周和24周样本与供者输注样本的相似性，通过Friedman检验，每个接受者的基线样本具有统计学意义。结论：这项研究表明，患者结肠细菌群的持久有益变化代表了健康供体的微生物群。操纵结肠微生物群以提高其保护和有益作用是治疗胃肠道疾病的新治疗策略的一个有前途的领域。
Goals: To determine whether fecal bacteriotherapy results in a durable beneficial change in the colonic microbiota of patients with flora-related disorders.Background: Earlier studies have implicated the colonic microbiota in a number of conditions. Administration of a fecal suspension from a healthy individual to an ill individual (fecal bacteriotherapy) can cure Clostridium difficile infection and potentially other diseases. Oral probiotics do not work in this condition, yet there has been no study to determine whether fecal bacteriotherapy results in prolonged implantation.Study: Fecal samples were collected from 10 patients undergoing fecal bacteriotherapy. Patients completed an antibiotic schedule and bowel lavage before the infusion of healthy donor feces. Using a molecular approach, the bacterial populations in patient fecal samples were followed from pretreatment to 24 weeks post-initial infusion and compared with the initial infused donor fecal suspension.Results: At intervals of 4, 8, and 24 weeks after the procedure, the bacterial populations in the patients' fecal samples consisted predominantly of bacteria derived from the healthy donor samples. Comparisons of similarity at 4, 8, and 24 week samples to the donor-infused sample were made and each recipient's baseline sample was statistically significant with Friedman test.Conclusions: This study demonstrates a durable beneficial change in the patients' bacterial populations of the colon to represent those of the healthy donor's microbiota. Manipulation of the colonic microbiota to improve its protective and beneficial role represents a promising field of new therapeutic strategies for the treatment of gastrointestinal conditions.