A constitutively active cryptochrome in Drosophila melanogaster
A constitutively active cryptochrome in Drosophila melanogaster
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DOI：
10.1038/nn1285
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发表时间：
2004-08-01
影响因子：
25
通讯作者：
Rosato, E
中科院分区：
文献类型：
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作者：
Dissel, S;Codd, V;Rosato, E
光激活隐花色素（CRY）调节果蝇昼夜光反应。在酵母中，去除羧基（C）末端以产生一种不依赖于光的组成型活性形式。在这里，我们表明，过度表达的果蝇有一个更长的自由运行期的运动活动，以及改变循环动力学的时钟蛋白的时间（TIM）和周期（PER）。此外，在细胞水平上，它们显示两个重要的行为起搏细胞群中TIM水平以及TIM和PER的核定位减少：大腹侧神经元和小腹侧神经元（LN（v）s）。这些影响是类似于野生型苍蝇在连续光照下看到的，并建议CRY的C末端的光敏，光解酶样蛋白质的一部分的调节作用。
Light-activated cryptochrome (CRY) regulates circadian photoresponses in Drosophila melanogaster. Removing the carboxy (C) terminus to create CRYDelta produces, in yeast, a light-independent, constitutively active form. Here we show that flies overexpressing CRYDelta have a longer free-running period of locomotor activity, as well as altered cycling kinetics of the clock proteins timeless (TIM) and period (PER). Moreover, at the cellular level, they show a reduction in the level of TIM and in the nuclear localization of TIM and PER in two significant clusters of behavioral pacemaker cells: the large and the small ventral lateral neurons (LN(v)s). These effects are similar to those seen in wild-type flies under continuous light and suggest a regulatory role for the C terminus of CRY on the photosensitive, photolyase-like part of the protein.