Protease-mediated ligation of abiotic oligomers.
Protease-mediated ligation of abiotic oligomers.
复制标题
蛋白酶介导的非生物寡聚物的连接。
DOI：
10.1021/ja055105m
复制
发表时间：
2005
影响因子：
15
通讯作者：
Kirshenbaum,Kent
中科院分区：
文献类型：
--
作者：
Yoo,Barney;Kirshenbaum,Kent
我们证明了蛋白水解酶可以催化模拟肽低聚物的连接。该酶被用来促进N-取代甘氨酸低聚物或类肽的天然连接。除了调节两个肽片段的有效缩合外，还进行了迭代连接事件，产生了分子量高达20 kDa的串联产物。有效地连接肽类折叠体可以使化学合成能够形成复杂三级结构的仿生大分子。
We demonstrate that proteases can catalyze the ligation of peptidomimetic oligomers. The enzyme clostripain was used to facilitate the native ligation of N-substituted glycine oligomers, or peptoids. In addition to mediating the efficient condensation of two peptoid fragments, iterative ligation events were also performed, giving rise to concatenation products with molecular weights up to 20 kDa. Efficient ligation of peptoid foldamers may enable the chemical synthesis of biomimetic macromolecules capable of forming complex tertiary structures.