Effects of Electroacupuncture at PC6 and ST36 on HeartRate Variability in Anesthetized Mice
Effects of Electroacupuncture at PC6 and ST36 on HeartRate Variability in Anesthetized Mice
复制标题
DOI：
10.15806/j.issn.2311-8571.2014.0015
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Xiao-Yu Wang;Wei He;Hong Shi;Hong-yan Shang;Yang-shuai Su;X. Jing
中科院分区：
文献类型：
--
作者：
Xiao-Yu Wang;Wei He;Hong Shi;Hong-yan Shang;Yang-shuai Su;X. Jing
目的：观察电针PC6、ST 36穴对麻醉小鼠心率变异性的影响，并比较其差异。方法：将33只C57 BL/6小鼠随机分为对照组、PC 6组和ST 36组，每组11只。心电图由两个针电极记录。对心率变异性数据进行时频分析，包括心率、R-R间期标准差和LF/HF比值。测试结果：在PC6电针期间，SDRR显著增加（P < 0.05），并在实验结束时维持在一个显著较高的水平，LF/HF比值在整个实验过程中逐渐增加。在ST 36电针时，SDRR显著增加（P < 0.05），LF/HF比值在整个实验过程中无明显变化趋势。与对照组相比，PC6组小鼠在电针刺激时心率显著下降（P < 0.01），并在整个实验过程中持续下降（P < 0.05）。时间谱分析显示，电针后对照组小鼠的SDRR显著低于PC 6组和ST 36组（P < 0.05）。电针后90 min，PC6组SDRR明显高于对照组（P < 0.01）。当在刺激下测量时，在PC6处的EA导致SDRR比ST 36显著增加。电针后90 min组与电针前比较差异有显著性（P < 0.01）。结论：电针PC6和ST 36对麻醉小鼠HRV下降有保护作用。与ST 36比较，电针PC6的作用更为显著，这是由于同一脊髓节段的节后纤维向心脏的交感神经活动增加所致。
Objective: To observe the change of heart rate variability in anesthetized mice after electroacupuncture on PC6 and ST36, and compare the difference between these points. Methods: A total of 33 C57BL/6 mice were randomly divided into control, PC6 and ST36 groups with 11 mice in each group. The electrocardiogram was recorded by two needle electrodes. The HRV data were analyzed by time and frequency analysis with heart rate, Standard Deviation of R-R Intervals and LF/HF Ratio. Result: During the EA at PC6, SDRR was significantly increased (P < 0.05) and maintained with a significantly higher level at the end of experiment, and the LF/HF ratio was gradually increased throughout the experiment. During the EA at ST36, SDRR was significantly increased (P < 0.05)and there wasno observable tendency of the change inLF/HF ratio duringthe experiment. In comparison with control,the HR of group PC6 mice decreased significantly during EA stimulation (P < 0.01) and continued declining throughout the experiment (P < 0.05). Time spectral analysis of the ECG recordings showed that control mice had significantly lower SDRR compared to group PC6 and group ST36 after EA stimulation(P < 0.05). SDRR was significantly increased when measured at 90mins after EA in group PC6 in comparison with control (P < 0.01). EA at PC6 caused a significant increase than ST36 in SDRR when measured at Stim. (P < 0.05) and 90mins after EA (P < 0.01). Conclusion: EA at PC6 and ST36 protected anesthesia mice against decline of HRV. In comparison with ST36, the effect of EA at PC6 was more significant, which was caused by the increase of the sympathetic nerve activities from the postganglionic fibers with the same spinal cord segments to heart.