IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects
IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects
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IL-10 基因修饰的人羊膜间充质干细胞通过多重协同效应增强再生伤口愈合
DOI：
10.1155/2019/9158016
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发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Wang, Dali
中科院分区：
文献类型：
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作者：
Xiao, Shune;Huang, Guangtao;Wang, Dali
间充质干细胞（MSCs）具有促进皮肤伤口愈合的能力。然而，MSCs的治疗效果似乎是有限的。修饰间充质干细胞靶基因以增加必要的生物学效应是一种很有前途的创面治疗策略。白细胞介素-10 （IL-10）是一种抗炎细胞因子，对伤口愈合有治疗作用。在本研究中，我们利用重组慢病毒载体修饰人羊膜间充质干细胞（hAMSCs）表达IL-10，并评价hAMSCs-IL-10在伤口愈合中的治疗作用。我们阐明了这些效应背后的机制。我们发现，促进伤口愈合是通过hAMSCs和IL-10的协同作用来维持的。与hAMSCs相比，hAMSCs- il -10在加速伤口愈合、促进血管生成、调节炎症、调节细胞外基质重塑等方面表现出更强的生物学效应。hAMSCs-IL-10能更好地促进创面愈合，提高愈合质量。这些数据可为临床应用hAMSCs-IL-10促进皮肤创面愈合和皮肤再生提供理论依据。
Mesenchymal stem cells (MSCs) possess a capacity to enhance cutaneous wound healing that is well characterized. However, the therapeutic effect of MSCs appears to be limited. Modifying MSC target genes to increase necessary biological effects is a promising strategy for wound therapy. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that has a therapeutic effect on wound healing. In this study, we modified human amniotic mesenchymal stem cells (hAMSCs) using recombinant lentiviral vectors for expressing IL-10 and evaluated the therapeutic effects of hAMSCs-IL-10 in wound healing. We elucidated the mechanisms underlying the effects. We found that promoting wound healing was maintained by synergistic effects of hAMSCs and IL-10. hAMSCs-IL-10 showed stronger biological effects in accelerating wound closure, enhancing angiogenesis, modulating inflammation, and regulating extracellular matrix remodeling than hAMSCs. hAMSCs-IL-10 would be better at promoting wound healing and improving healing quality. These data may provide a theoretical foundation for clinical administration of hAMSCs-IL-10 in cutaneous wound healing and skin regeneration.