INHBA transfection regulates proliferation, apoptosis and hormone synthesis in sheep granulosa cells
INHBA transfection regulates proliferation, apoptosis and hormone synthesis in sheep granulosa cells
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INHBA转染调节绵羊颗粒细胞增殖、凋亡和激素合成
DOI：
10.1016/j.theriogenology.2021.09.004
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发表时间：
2021-09-16
期刊：
影响因子：
2.8
通讯作者：
Wang, Feng
中科院分区：
文献类型：
--
作者：
Bao, Yongjin;Yao, Xiaolei;Wang, Feng
抑制素亚基 β A (INHBA) 参与抑制素 A、激活素 A 和激活素 AB 的合成。在这里，我们通过体外转染研究了INHBA对绵羊颗粒细胞（GC）增殖、凋亡和激素合成的影响和分子机制。我们首先注意到INHBA表达随着卵泡直径的增加而增加，并且广泛分布于卵巢组织中。与阴性对照相比，随着INHBA的过表达和沉默，GC的增殖率分别显着增加和减少。 INHBA转染影响GC增殖和凋亡，调节许多细胞周期相关和凋亡相关基因的表达。 INHBA 过表达显着降低了激活素和雌二醇的分泌，同时增加了抑制素和孕酮的分泌。促卵泡激素β亚基的表达分别随着INHBA过表达和敲低而显着降低和增加。值得注意的是，INHBA 的沉默抑制了许多转化生长因子β相关基因的表达。总体而言，INHBA基因的功能分子可能通过调节绵羊GC的增殖、凋亡和卵泡发生相关激素的分泌而与卵泡发育相关。此外，我们的发现可能有助于更好地了解卵泡发育规律，从而提高雌性动物的生殖性能。 (c) 2021 年由爱思唯尔公司出版
Inhibin subunit beta A (INHBA) participates in the synthesis of inhibin A, activin A and activin AB. Here we investigated the effect and molecular mechanism of INHBA on proliferation, apoptosis and hormone synthesis in sheep granulosa cells (GCs) using in vitro transfection. We first noticed that INHBA expression increased with follicle diameter and was widely distributed in ovarian tissue. The prolifer-ation rate of GCs was significantly increased and decreased with overexpression and silence of INHBA, respectively, compared with the negative controls. INHBA transfection affected GC proliferation and apoptosis, regulating the expression of many cell cycle-related and apoptosis-related genes. INHBA overexpression significantly decreased activin and estradiol secretion while increasing inhibin and progesterone secretion. The expression of follicle-stimulating hormone beta subunit was significantly decreased and increased with INHBA overexpression and knockdown, respectively. Notably, silence of INHBA inhibited the expression of many transforming growth factor beta-related genes. Overall, the functional molecule of INHBA gene may be associated with follicular development via regulating pro-liferation, apoptosis and folliculogenesis-related hormone secretion of sheep GCs. In addition, our findings may contribute to a better understanding of the law of follicular development and thus improve the reproductive performance of female animals. (c) 2021 Published by Elsevier Inc.