Genetic variability of human metapneumovirus amongst an all ages population in Cambodia between 2007 and 2009
Genetic variability of human metapneumovirus amongst an all ages population in Cambodia between 2007 and 2009
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DOI：
10.1016/j.meegid.2011.01.016
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发表时间：
2013-04-01
影响因子：
3.2
通讯作者：
Buchy, Philippe
中科院分区：
文献类型：
--
作者：
Arnott, Alicia;Vong, Sirenda;Buchy, Philippe
人偏肺病毒（human metapneumovirus，HMPV）是2001年发现的一种新型急性呼吸道感染病原体。HMPV的再感染很常见，目前还没有针对HMPV感染的疫苗。已经鉴定了HMPV的两种基因型A和B，这两种基因型都可以进一步分为至少两种不同的亚基因型。在这里，我们报告的第一项研究的结果，调查在柬埔寨境内传播的HMPV毒株的遗传变异。2007年至2009年期间，柬埔寨连续3年因ALRI住院的所有年龄段患者人群中HMPV感染的总体发生率为1.7%。HMPV感染在5岁以下儿童中的发病率最高，肺炎或支气管肺炎是所有年龄组中最常见的临床诊断。HMPV感染的发病率每年都有变化。正如预期的那样，保守的F基因序列之间的遗传多样性是低的，但非常高的G基因序列之间，一些菌株共享低至56.3%和34.2%的同源性在核苷酸和氨基酸水平，分别。在2个地理位置不同的省级医院招募的患者中，检测到属于HMPV亚型B1、B2和谱系A2b的菌株的同时共循环。亚基因型B2菌株负责检测到的大多数感染，并观察到谱系A2b菌株感染和疾病严重程度之间的显著（p = 0.013）关联。(C)2011 Elsevier B.V.保留所有权利。
First identified in 2001, human metapneumovirus (HMPV) is a novel pathogen and causative agent of acute respiratory tract infection. Re-infection with HMPV is common, and currently there is no available vaccine against HMPV infection. Two genotypes of HMPV have been identified, A and B, both of which can be divided further into at least two distinct sub-genotypes. Here we report the results of the first study to investigate the genetic variability of HMPV strains circulating within Cambodia. The overall incidence of HMPV infection amongst an all-ages population of patients hospitalised with ALRI in Cambodia during 3 consecutive years, between 2007 and 2009, was 1.7%. The incidence of HMPV infection was highest amongst children less than 5 years of age, with pneumonia or bronchopneumonia the most frequent clinical diagnoses across all age groups. The incidence of HMPV infection varied annually. As anticipated, genetic diversity was low amongst the conserved F gene sequences but very high amongst G gene sequences, some strains sharing as little as 56.3% and 34.2% homology at the nucleotide and amino acid levels, respectively. Simultaneous co-circulation of strains belonging to the HMPV sub-genotypes B1, B2 and lineage A2b, amongst patients recruited at 2 geographically distinct provincial hospitals, was detected. Sub-genotype B2 strains were responsible for the majority of the infections detected, and a significant (p = 0.013) association between infection with lineage A2b strains and disease severity was observed. (C) 2011 Elsevier B.V. All rights reserved.