Synthetic Bone-Like Structures Through Omnidirectional Ceramic Bioprinting in Cell Suspensions
Synthetic Bone-Like Structures Through Omnidirectional Ceramic Bioprinting in Cell Suspensions
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DOI：
10.1002/adfm.202008216
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发表时间：
2021-01-20
影响因子：
19
通讯作者：
Roohani, Iman
中科院分区：
文献类型：
--
作者：
Romanazzo, Sara;Molley, Thomas Gregory;Roohani, Iman
在组织工程支架内整合分级结构、化学和功能活性在模拟天然骨组织方面非常重要。骨是一种高度矿化的组织，其在环境条件下通过在骨驻留细胞存在下有机基质内的矿物相的连续结晶而形成。尽管最近在复杂的工程组织的生物支架方面取得了进展，但骨微环境的异质性的复制一直是构建仿生骨支架的主要挑战。在本文中，受骨生物矿化过程的启发，通过在具有活细胞的支持性微凝胶基质中3D写入新型磷灰石转化油墨来展示骨模拟构建体的第一个实例。使用这种技术，可以在室温下制造复杂的仿骨结构，无需侵入性化学物质、辐射或后处理步骤。这项研究表明，矿化结构可以沉积在一个高密度的干细胞，指导细胞组织，并促进体外成骨。这些发现为制造用于骨组织再生的骨模拟结构提供了一种新的策略，其范围为生成用于疾病建模、多细胞递送和体内骨修复的定制骨微环境。
The integration of hierarchical structure, chemistry, and functional activity within tissue-engineered scaffolds is of great importance in mimicking native bone tissue. Bone is a highly mineralized tissue which forms at ambient conditions by continuous crystallization of the mineral phase within an organic matrix in the presence of bone residing cells. Despite recent advances in the biofabrication of complex engineered tissues, replication of the heterogeneity of bone microenvironments has been a major challenge in constructing biomimetic bone scaffolds. Herein, inspired by the bone biomineralization process, the first example of bone mimicking constructs by 3D writing of a novel apatite-transforming ink in a supportive microgel matrix with living cells is demonstrated. Using this technique, complex bone-mimicked constructs are made at room temperature without requiring invasive chemicals, radiation, or postprocessing steps. This study demonstrates that mineralized constructs can be deposited within a high density of stem cells, directing the cellular organization, and promoting osteogenesis in vitro. These findings offer a new strategy for fabrication of bone mimicking constructs for bone tissue regeneration with scope to generate custom bone microenvironments for disease modeling, multicellular delivery, and in vivo bone repair.