Protective effects of patchouli alcohol isolated from Pogostemon cablin on lipopolysaccharide-induced acute lung injury in mice
Protective effects of patchouli alcohol isolated from Pogostemon cablin on lipopolysaccharide-induced acute lung injury in mice
复制标题
广藿香醇对脂多糖诱导的小鼠急性肺损伤的保护作用
DOI：
10.3892/etm.2015.2918
复制
发表时间：
2016-02-01
影响因子：
2.7
通讯作者：
Li, Yucui
中科院分区：
文献类型：
--
作者：
Su, Zuqing;Liao, Jinbin;Li, Yucui
广藿香醇 (PA) 是从广藿香中分离出来的三环倍半萜，具有抗炎、抗流感和增强认知的生物活性。本研究旨在探讨PA对气管内滴注脂多糖（LPS）所致小鼠急性肺损伤（ALI）的保护作用。地塞米松被用作预防 LPS 诱发的 ALI 的阳性药物。本研究结果表明，PA 预处理可显着提高 ALI 小鼠的存活率，减轻组织病理学损伤和肺水肿，并降低支气管肺泡灌洗液 (BALF) 中的蛋白质含量。此外，PA显着抑制BALF中促炎细胞因子的表达水平，包括肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6，下调髓过氧化物酶和丙二醛的水平，上调肺组织中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性水平。这些结果表明，PA可能对LPS诱导的小鼠ALI发挥有效的保护作用，其机制可能与PA的抗炎和抗氧化活性有关。
Patchouli alcohol (PA) is a tricyclic sesquiterpene isolated from Pogostemon cablin, which exerts anti-inflammatory, anti-influenza and cognitive-enhancing bioactivities. The present study aimed to investigate the protective effects of PA on acute lung injury (ALI) induced by intratracheal instillation of lipopolysaccharide (LPS) in mice. Dexamethasone was used as a positive drug for protection against LPS-induced ALI. The results of the present study demonstrated that pretreatment with PA significantly increased survival rate, attenuated histopathologic damage and lung edema, and decreased the protein content in the bronchoalveolar lavage fluid (BALF) of mice with ALI. Furthermore, PA significantly inhibited the expression levels of proinflammatory cytokines, including tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 in the BALF, downregulated the levels of myeloperoxidase and malondialdehyde, and upregulated the activity levels of superoxide dismutase and glutathione peroxidase in lung tissue. These results indicated that PA may exert potent protective effects against LPS-induced ALI in mice, the mechanisms of which are possibly associated with the anti-inflammatory and antioxidative activities of PA.