Porcine enamel matrix derivative enhances trabecular bone regeneration during wound healing of injured rat femur
Porcine enamel matrix derivative enhances trabecular bone regeneration during wound healing of injured rat femur
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DOI：
10.1002/ar.10016
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发表时间：
2001-12-01
影响因子：
--
通讯作者：
Sasaki, T
中科院分区：
文献类型：
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作者：
Kawana, F;Sawae, Y;Sasaki, T
为了阐明釉基质衍生物（EMD：Emdogain（R））对大鼠股骨钻孔损伤后骨再生的影响，骨缺损用EMD或其载体PGA填充作为对照。术后第4 ~ 28天，通过各种形态学方法对解剖的股骨进行检查。在两个实验组中，术后第7天，在圆柱形骨缺损的髓腔内形成了骨小梁（骨唾液酸蛋白（BSP）免疫染色）。在这些新形成的BSP阳性骨小梁上清楚地观察到立方成骨细胞。在第7天和第14天，这些骨小梁上出现了许多强表达组织蛋白酶K的多核巨细胞，表明骨重建活跃。在这些骨小梁中，Ca和P重量%和Ca/P比与皮质骨相似，PGA和EMD应用组之间无显著差异。术后第7天，EMD应用组的新形成骨小梁的骨体积分数显著高于PGA应用对照组。然而，由于骨重建活跃和骨体积显著减少，在术后第14天和第28天，实验组之间的松质骨体积分数不再有显著差异。我们的研究结果表明，EMD具有促进骨和骨髓再生损伤长骨愈合过程中的骨。Anat Ree 264：438-446，2001年。(C)2001 Wiley-Liss，Inc.
To elucidate the effects of enamel matrix derivative (EMD: Emdogain(R)) on bone regeneration in rat femurs after drill-hole injury, defects in bone were filled with either EMD or its carrier, PGA, as control. On postoperative days 4 to 28, dissected femurs were examined by means of various morphological approaches. In both experimental groups, formation of trabecular bone, which was immunostained for bone sialoproteins (BSP), had occurred in the medullary cavities at cylindrical bone defects on Day 7 postoperatively. Cuboidal osteoblasts were clearly observed on these newly-formed BSP-positive bone trabeculae. On Days 7 and 14, many multinucleated giant cells, which strongly expressed cathepsin K, had appeared on these bone trabeculae, indicating active bone remodeling. In these bone trabeculae, Ca and P weight % and Ca/P ratio were similar to those of cortical bone, and there was no significant difference between the PGA- and EMD-applied groups. Bone volume fraction of newly-formed bone trabeculae on Day 7 postoperatively was significantly higher in the EMD-applied group than in the PGA-applied controls. Because of active bone remodeling and the marked decrease of bone volume, on Days 14 and 28 postoperatively, however, there was no longer a significant difference in trabecular bone volume fraction between the experimental groups. Our results suggest that EMD possesses an osteo-promotive effect on bone and medullary regeneration during wound healing of injured long bones. Anat Ree 264:438-446, 2001. (C) 2001 Wiley-Liss, Inc.