The Chromatin of Candida albicans Pericentromeres Bears Features of Both Euchromatin and Heterochromatin.
The Chromatin of Candida albicans Pericentromeres Bears Features of Both Euchromatin and Heterochromatin.
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DOI：
10.3389/fmicb.2016.00759
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发表时间：
2016
影响因子：
5.2
通讯作者：
Buscaino A
中科院分区：
文献类型：
--
作者：
Freire-Benéitez V;Price RJ;Buscaino A
着丝粒是着丝粒组装的场所，对染色体的稳定性和完整性非常重要。大多数真核生物具有由组蛋白H3变体CENP-A的存在表观遗传地指定的区域着丝粒。CENP-A染色质通常被包装成转录沉默异染色质的着丝粒周围区域包围。白色念珠菌是最常见的人类真菌病原体，具有组装成CENP-A染色质的小区域着丝粒。C.白色念珠菌的着丝粒周围区域未知。在这里，我们第一次提出这个问题。我们发现C.白色念珠菌的近着丝粒组装成具有常染色质和异染色质特征的中间染色质状态。着丝粒周围染色质与高度乙酰化的核小体相关，如在基因组的常染色质区域中发现的;和在H3 K4上低甲基化的，如在异染色质中发现的。这种中间染色质状态抑制转录，并部分抑制近端基因和插入的标记基因的表达。我们的分析确定了一个新的染色质状态与近着丝粒区域。
Centromeres, sites of kinetochore assembly, are important for chromosome stability and integrity. Most eukaryotes have regional centromeres epigenetically specified by the presence of the histone H3 variant CENP-A. CENP-A chromatin is often surrounded by pericentromeric regions packaged into transcriptionally silent heterochromatin. Candida albicans, the most common human fungal pathogen, possesses small regional centromeres assembled into CENP-A chromatin. The chromatin state of C. albicans pericentromeric regions is unknown. Here, for the first time, we address this question. We find that C. albicans pericentromeres are assembled into an intermediate chromatin state bearing features of both euchromatin and heterochromatin. Pericentromeric chromatin is associated with nucleosomes that are highly acetylated, as found in euchromatic regions of the genome; and hypomethylated on H3K4, as found in heterochromatin. This intermediate chromatin state is inhibitory to transcription and partially represses expression of proximal genes and inserted marker genes. Our analysis identifies a new chromatin state associated with pericentromeric regions.