Expression of bone microsomal casein kinase II, bone sialoprotein, and osteopontin during the repair of calvarial defects
Expression of bone microsomal casein kinase II, bone sialoprotein, and osteopontin during the repair of calvarial defects
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DOI：
10.1016/s8756-3282(98)00057-x
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发表时间：
1998-06-01
期刊：
影响因子：
4.1
通讯作者：
Salih, E
中科院分区：
文献类型：
--
作者：
Wang, J;Glimcher, MJ;Salih, E
在植入脱钙大鼠骨基质诱导的大鼠颅骨缺损修复过程中，从第 2 天到第 21 天绘制了骨微粒体酪蛋白激酶 II、骨桥蛋白、骨唾液蛋白、碱性磷酸酶的时间表达和固体钙-无机正磷酸盐矿物相的积累。与皮下或肌内植入相同的脱钙骨基质时发生的事件顺序不同，在这种情况下，响应植入物而形成的第一个组织是软骨，随后钙化，然后再吸收并被骨替代，而颅骨缺损的修复则完全不同。在后一种情况下，诱导的第一个细胞是未分化的间充质细胞和早期成纤维细胞，然后是成骨细胞直接骨形成。稍后，形成了一些小软骨岛，它们与新形成的骨基质广泛分离并在空间上不同。到第 21 天，所有软骨和大部分植入的脱钙骨基质随后被吸收并被新骨取代。这种通过直接骨形成修复骨缺损的体内模型提供了一个极好的系统来跟踪特定的生化和物理化学事件。矿物质和两种非胶原磷蛋白（骨唾液蛋白和骨桥蛋白）的总积累和积累速率，以及碱性磷酸酶的活性，以及首次在体内或细胞培养中确定了微粒体酪蛋白激酶II（磷酸化骨磷蛋白的主要酶）的活性，作为体内愈合时间的函数，磷蛋白和磷酸钙矿物质相积累的总体模式及其关系与成骨细胞培养物中报告的类似，也作为时间函数进行监测，(C) 1998，Elsevier Science Inc. 保留所有权利。
The temporal expression of bone microsomal casein kinase II, osteopontin, bone sialoprotein, alkaline phosphatase, and the accumulation of a solid calcium-inorganic orthophosphate mineral phase, have been charted from day 2 to day 21 during the repair of calvarial defects in rats induced by the implantation of decalcified rat bone matrix. Unlike the sequence of events that occur when the same decalcified bone matrix is implanted subcutaneously or intramuscularly, in which cases the first tissue to form in response to the implant is cartilage that subsequently calcifies and is later resorbed and replaced by bone, the repair of cranial defects is quite different, In the latter case, the first cells induced are undifferentiated mesenchymal tells and early fibroblasts followed by osteoblastic direct bone formation. Somewhat later a few small islands of cartilage are formed, widely separated and spatially distinct from the newly formed bone matrix, All of the cartilage and most of the implanted decalcified bone matrix are later resorbed and replaced by new bone by day 21., This in vivo model of the repair of a bone defect by direct bone formation has provided an excellent system to follow specific biochemical and physicochemical events. The total accumulation and rate of accumulation of the mineral and the two noncollagenous phosphoproteins (hone sialoprotein and osteopontin), as well as the activities of alkaline phosphatase, and for the first time either in vivo or in cell culture, the activity of microsomal casein kinase II, the major enzyme that phosphorylates the bone phosphoproteins, have been determined as a function of healing time in vivo, The overall general pattern of accumulation of the phosphoproteins and calcium-phosphate mineral phase and their relationships are similar to those reported in osteoblast cell cultures also monitored as a function of time, (C) 1998 by Elsevier Science Inc. All rights reserved.