Polar localization of CheO under hypoxia promotes Campylobacter jejuni chemotactic behavior within host.
Polar localization of CheO under hypoxia promotes Campylobacter jejuni chemotactic behavior within host.
复制标题
缺氧条件下 CheO 的极性定位促进宿主内空肠弯曲杆菌的趋化行为
DOI：
10.1371/journal.ppat.1010953
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发表时间：
2022-11
期刊：
影响因子：
6.7
通讯作者：
中科院分区：
文献类型：
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作者：
空肠弯曲杆菌是一种全球关注的食源性人畜共患病病原体，也是细菌性腹泻病的主要病因。与其他肠道病原体不同，空肠弯曲菌对生长和营养要求严格，但缺乏许多为致病或与宿主相互作用而进化的毒力因子。目前还不清楚这种细菌如何适应肠道生活方式。在这里，我们发现了Cheo蛋白(CJJ81176_1265)是空肠弯曲菌在小鼠肠道中定植所必需的，它通过在限氧环境中对鞭毛旋转的化学趋化控制而发挥作用。CHEO与趋化信号蛋白CHEA和CHEZ相互作用，也与鞭毛转子成分FliM和FliY相互作用。在微氧条件下，Cheo定位于空肠弯曲菌中化学感觉阵列和鞭毛机械所在的细胞极点，其极性定位依赖于化学感觉阵列的形成。介导能量趋化的几个化学受体协调决定了CHEO的两极分布。对一个ΔCHIO突变体的抑制子筛选发现，FliM中的单个残基变异可以减轻由于缺少CHIO而引起的表型，证实了它在鞭毛转子开关中的调节作用。CHEO同源物只存在于弯曲杆菌门的物种中，主要是寄主相关的弯曲杆菌属、螺杆菌和Wolinella属的物种。CHEO的结果为了解空肠弯曲菌和近缘物种趋化信号转导的复杂性提供了深入的见解。重要的是，在化学感觉阵列中招募Cheo以促进低氧下的趋化行为，代表了空肠弯曲菌对人和动物肠道的一种新的适应策略。
Campylobacter jejuni is a food-borne zoonotic pathogen of worldwide concern and the leading cause of bacterial diarrheal disease. In contrast to other enteric pathogens, C. jejuni has strict growth and nutritional requirements but lacks many virulence factors that have evolved for pathogenesis or interactions with the host. It is unclear how this bacterium has adapted to an enteric lifestyle. Here, we discovered that the CheO protein (CJJ81176_1265) is required for C. jejuni colonization of mice gut through its role in chemotactic control of flagellar rotation in oxygen-limiting environments. CheO interacts with the chemotaxis signaling proteins CheA and CheZ, and also with the flagellar rotor components FliM and FliY. Under microaerobic conditions, CheO localizes at the cellular poles where the chemosensory array and flagellar machinery are located in C. jejuni and its polar localization depends on chemosensory array formation. Several chemoreceptors that mediate energy taxis coordinately determine the bipolar distribution of CheO. Suppressor screening for a ΔcheO mutant identified that a single residue variation in FliM can alleviate the phenotype caused by the absence of CheO, confirming its regulatory role in the flagellar rotor switch. CheO homologs are only found in species of the Campylobacterota phylum, mostly species of host-associated genera Campylobacter, Helicobacter and Wolinella. The CheO results provide insights into the complexity of chemotaxis signal transduction in C. jejuni and closely related species. Importantly, the recruitment of CheO into chemosensory array to promote chemotactic behavior under hypoxia represents a new adaptation strategy of C. jejuni to human and animal intestines.
影响因子：
3.4
作者：
Mueller, Axel;Beeby, Morgan;McDowall, Alasdair W.;Chow, Janet;Jensen, Grant J.;Clemons, William M., Jr.
通讯作者：
Clemons, William M., Jr.