THE COMPLETE SEQUENCE OF A FULL LENGTH CDNA FOR HUMAN-LIVER GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - EVIDENCE FOR MULTIPLE MESSENGER-RNA SPECIES
THE COMPLETE SEQUENCE OF A FULL LENGTH CDNA FOR HUMAN-LIVER GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - EVIDENCE FOR MULTIPLE MESSENGER-RNA SPECIES
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DOI：
10.1093/nar/12.23.9179
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
SALVATORE, F
中科院分区：
文献类型：
--
作者：
ARCARI, P;MARTINELLI, R;SALVATORE, F
已鉴定出含有来自人胎肝mRNA的编码甘油醛-3-磷酸脱氢酶的65 bp cDNA片段的重组M13克隆（042），并已将其用于从全长人成人肝cDNA文库中分离重组克隆pG 1，该克隆已在M13噬菌体中亚克隆并用链终止子法完全测序。除了1008 bp的编码区外，cDKA序列在5′端包括60个核苷酸，在3′端包括204个核苷酸，直到polyA尾。pG 1与人肝总RNA的杂交仅显示一条与pG 1 cDNA大小相当的条带。与人肝癌和肾癌细胞株RNA的杂交信号更强，克隆042与克隆pG 1的同源区序列同源性为86%。另一方面，翻译序列与已知的人肌肉蛋白序列的同源性在77%至90%之间，这些数据表明存在一个以上的基因编码的G3 PD。人DNA的Southern印迹，用几种限制性内切酶消化也表明，在人类基因组中存在几个同源序列。
A recombinant M13 clone (042) containing a 65 b.p. cDNA fragment from human fetal liver mRNA coding for glyceraldehyde-3-phosphate dehydrogenase has been identified and it has been used to isolate from a full-length human adult liver cDNA library a recombinant clone, pGl, which has been subcloned in M13 phage and completely sequenced with the chain terminator method. Besides the coding region of 1008 b.p., the cDKA sequence includes 60 nucleotides at the 5′-end and 204 nucleotides at the 3′-end up to the polyA tail. Hybridization of pGl to human liver total RNA shows only one band about the size of pGl cDNA. A much stronger hybridization signal was observed using RNA derived from human hepatocarcinoma and kidney carcinoma cell lines.Sequence homology between clone 042 and the homologous region of clone pGl is 86%. On the other hand, homology among the translated sequences and the known human muscle protein sequence ranges between 77 and 90% these data demonstrate the existence of more than one gene coding for G3PD.Southern blot of human DNA, digested with several restriction enzymes, also indicate that several homologous sequences are present in the human genoma.