Full genome-based characterization of G4P[6] rotavirus strains from diarrheic patients in Thailand: Evidence for independent porcine-to-human interspecies transmission events
Full genome-based characterization of G4P[6] rotavirus strains from diarrheic patients in Thailand: Evidence for independent porcine-to-human interspecies transmission events
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DOI：
10.1007/s11262-021-01851-y
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发表时间：
2021-06-09
期刊：
影响因子：
1.6
通讯作者：
Komoto，Satoshi
中科院分区：
文献类型：
--
作者：
Tacharoenmuang，Ratana;Guntapong，Ratigorn;Komoto，Satoshi
人G4 P轮状病毒株的确切进化模式[6]仍有待阐明。这些菌株具有独特的和菌株特异性的基因型星座，提出了一个问题，即G4 P [6]菌株主要是通过独立的种间传播还是在种间传播后通过人传人传播。在泰国严重腹泻住院患者的粪便标本中鉴定出两种G4 P [6]轮状病毒株，即DU 2014 -259（RVA/Human-wt/THA/DU 2014 -259/2014/G4 P [6]）和PK 2015 -1-0001（RVA/Human-wt/THA/PK 2015 -1-0001/2015/G4 P [6]）。在这里，我们分析了两种人类G4 P菌株的全基因组，这为研究和确认它们的进化起源提供了机会。在全基因组分析中，两种菌株均表现出独特的Wa样基因型星座G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1。NSP 1基因型A8常见于猪轮状病毒株中。此外，在系统发育分析中，菌株DU 2014 -259和PK 2015 -1-0001的11个基因中的每一个似乎都是猪源的。另一方面，两种研究菌株在11个基因片段中的9个（VP 4、VP 6、VP 1-VP 3和NSP 2-NSP 5）上始终形成不同的簇，强烈表明发生了独立的猪-人种间传播事件。我们的观察为了解人畜共患G4 P [6]毒株的起源以及猪和人轮状病毒毒株之间的动态相互作用提供了重要见解。
The exact evolutionary patterns of human G4P[6] rotavirus strains remain to be elucidated. Such strains possess unique and strain-specific genotype constellations, raising the question of whether G4P[6] strains are primarily transmitted via independent interspecies transmission or human-to-human transmission after interspecies transmission. Two G4P[6] rotavirus strains were identified in fecal specimens from hospitalized patients with severe diarrhea in Thailand, namely, DU2014-259 (RVA/Human-wt/THA/DU2014-259/2014/G4P[6]) and PK2015-1-0001 (RVA/Human-wt/THA/PK2015-1-0001/2015/G4P[6]). Here, we analyzed the full genomes of the two human G4P[6] strains, which provided the opportunity to study and confirm their evolutionary origin. On whole genome analysis, both strains exhibited a unique Wa-like genotype constellation of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The NSP1 genotype A8 is commonly found in porcine rotavirus strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strains DU2014-259 and PK2015-1-0001 appeared to be of porcine origin. On the other hand, the two study strains consistently formed distinct clusters for nine of the 11 gene segments (VP4, VP6, VP1-VP3, and NSP2-NSP5), strongly indicating the occurrence of independent porcine-to-human interspecies transmission events. Our observations provide important insights into the origin of zoonotic G4P[6] strains, and into the dynamic interaction between porcine and human rotavirus strains.