EFFECT OF ACTINOMYCIN D AND GUANIDINE ON FORMATION OF A RIBONUCLEIC ACID POLYMERASE INDUCED BY FOOT-AND-MOUTH-DISEASE VIRUS AND ON REPLICATION OF VIRUS AND VIRAL RIBONUCLEIC ACID
EFFECT OF ACTINOMYCIN D AND GUANIDINE ON FORMATION OF A RIBONUCLEIC ACID POLYMERASE INDUCED BY FOOT-AND-MOUTH-DISEASE VIRUS AND ON REPLICATION OF VIRUS AND VIRAL RIBONUCLEIC ACID
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DOI：
10.1042/bj1120317
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发表时间：
1969-01-01
影响因子：
4.1
通讯作者：
BROWN, F
中科院分区：
文献类型：
--
作者：
BLACK, DN;BROWN, F
口蹄疫病毒感染幼仓鼠肾细胞后，60分钟即可检测到依赖RNA的RNA聚合酶。感染后60 min。在病毒RNA合成开始之前。首次检测到聚合酶的时间与放线菌素D（50μg/ml）107细胞）或胍（1 mg./ 107细胞）抑制病毒复制。然而，通过增加胍的浓度，可以在生长周期的后期抑制病毒复制，从而对胍特异性作用于病毒RNA聚合酶形成的假设的有效性产生怀疑。
The RNA-dependent RNA polymerase induced in baby-hamster kidney cells by infection with foot-and-mouth-disease virus can be detected as early as 60min. after infection, which is 60min. before viral RNA synthesis commences. The time at which the polymerase can first be detected coincides with the latest time at which actinomycin D (50μg./107cells) or guanidine (1mg./107cells) inhibits virus replication. However, by increasing the concentration of guanidine, viral replication can be inhibited later in the growth cycle, casting doubt on the validity of the hypothesis that guanidine acts specifically on the formation of the viral RNA polymerase.