Lactococcus hircilactis sp nov and Lactococcus laudensis sp nov., isolated from milk
Lactococcus hircilactis sp nov and Lactococcus laudensis sp nov., isolated from milk
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DOI：
10.1099/ijs.0.000225
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发表时间：
2015-07-01
影响因子：
2.8
通讯作者：
Giraffa, Giorgio
中科院分区：
文献类型：
--
作者：
Meucci, Aurora;Zago, Miriam;Giraffa, Giorgio
分别从意大利瓦尔泰利纳 (Valtellina) 的羊奶和意大利特罗姆皮亚 (Valle Trompia) 的牛奶中分离出两种乳酸菌菌株，命名为 117(T) 和 4195(T)，并通过多相方法进行分类学表征。这些菌株是革兰氏染色阳性、球状、不形成孢子和过氧化氢酶阴性的细菌。形态学、生理学和系统发育数据表明这些分离株属于乳球菌属。菌株117(T)与福建乳球菌、乳酸乳球菌亚种密切相关。乳酸乳球菌，乳酸乳球菌亚种。乳脂，乳酸乳球菌亚种。霍德尼亚埃，乳酸乳球菌亚种。 tructae 和 Lactococcus taiwanensis 的 16S rRNA 和 rpoB 基因序列相似性分别为 93-94% 和 82-89%。菌株4195(T)与中港乳球菌、棉子乳酸乳球菌、植物乳球菌和鱼乳球菌密切相关，16S rRNA和rpoB基因序列相似性分别为92-98%和86-99%。根据这一证据和本研究中获得的数据，牛奶分离物代表了乳球菌属的两个新物种，其名称为 Lactococcus hircilactis sp。 nov.，和劳登乳球菌 sp。十一月被提议。各自的类型菌株是117(T)(＝LMG 28352(T)＝DSM 28960(T))和4195(T)(＝LMG 28353(T)＝DSM 28961(T))。
Two strains of lactic acid bacteria, designated 117(T) and 4195(T), were isolated from goat milk in Valtellina, Italy and from cow milk in Valle Trompia, Italy, respectively, and characterized taxonomically by a polyphasic approach. The strains were Gram-stain-positive, coccoid, non-spore-forming and catalase-negative bacteria Morphological, physiological and phylogenetic data indicated that these isolates belonged to the genus Lactococcus. Strain 117(T) was closely related to Lactococcus fujiensis, Lactococcus lactis subsp. lactis, L. lactis subsp. cremoris, L. lactis subsp. hordniae, L. lactis subsp. tructae and Lactococcus taiwanensis, showing 93-94% and 82-89% 16S rRNA and rpoB gene sequence similarities, respectively. Strain 4195(T) was closely related to Lactococcus chungangensis, Lactococcus raffinolactis, Lactococcus plantarum and Lactococcus piscium, showing 92-98% and 86-99% 16S rRNA and rpoB gene sequence similarities, respectively. Based on this evidence and the data obtained in the present study, the milk isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus hircilactis sp. nov., and Lactococcus laudensis sp. nov. are proposed. The respective type strains are 117(T) (=LMG 28352(T)=DSM 28960(T)) and 4195(T) (=LMG 28353(T)=DSM 28961(T)).