The LIM-homeodomain protein Lhx2 is required for complete development of mouse olfactory sensory neurons
The LIM-homeodomain protein Lhx2 is required for complete development of mouse olfactory sensory neurons
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DOI：
10.1073/pnas.0400940101
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发表时间：
2004-06-08
影响因子：
11.1
通讯作者：
Mombaerts, P
中科院分区：
文献类型：
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作者：
Hirota, J;Mombaerts, P
在小鼠中，大约有1,000个气味受体（OR）基因在嗅觉感觉神经元（OSN）中表达。同源结构域位点可以在几个OR基因的启动子和上游区域中被识别。在这里，使用酵母单杂交系统和电泳迁移率变动分析，我们报告Lhx 2，一个LIM同源结构域蛋白，结合到小鼠M71 OR启动子区的同源结构域位点。在Lhx 2缺陷型小鼠中，嗅上皮的形态大体正常。然而，OMP的表达被消除，GAP 43的表达严重减少，表明没有产生成熟的OSN，并且产生很少的不成熟的OSN。M71和其他OR基因也不表达。OSN的发育似乎在终末分化为神经元和过渡为未成熟神经元之间被阻止。因此，Lhx 2是小鼠OSN完全发育所需的。
In mice, approximate to1,000 odorant receptor (OR) genes are expressed in olfactory sensory neurons (OSNs). Homeodomain sites can be recognized in the promoter and upstream regions of several OR genes. Here, using the yeast one-hybrid system and electrophoretic mobility shift assay, we report that Lhx2, a LIM-homeodomain protein, binds to the homeodomain site in the mouse M71 OR promoter region. In Lhx2-deficient mice, the morphology of the olfactory epithelium is grossly normal. However, expression of OMP is abolished and that of GAP43 is severely reduced, indicating that no mature and few immature OSNs are produced. M71 and other OR genes also are not expressed. OSN development appears to be arrested between the terminal differentiation into neurons and the transition to immature neurons. Thus, Lhx2 is required for complete development of OSNs in mice.