Pyrene degradation by Mycobacterium sp. strain KR2.

Pyrene degradation by Mycobacterium sp. strain KR2.
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DOI:
10.1016/s0045-6535(97)10240-5
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发表时间:
1998-06
期刊:
影响因子:
8.8
通讯作者:
K. Rehmann;Harald Noll;Christian E. W. Steinberg;A. Kettrup
K. Rehmann;Harald Noll;Christian E. W. Steinberg;A. Kettrup
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
K. Rehmann;Harald Noll;Christian E. W. Steinberg;A. Kettrup

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从多环芳烃(PAH)污染的土壤中分离到一株能以芘为唯一碳源和能源的分枝杆菌KR2菌株。在20℃的温度下,该菌株在8d内代谢了高达60%的加入量(0.5 mg/mL),降解产物为顺-4,5-芘二氢二醇、4,5-菲二酸、1-羟基-2-萘甲酸、2-羧基苯甲醛、邻苯二甲酸和原儿茶酸。基于这些发现,提出了一种芘的降解途径,这与迄今为止发表的关于细菌芘代谢的数据很好地一致。
A Mycohacterium sp., strain KR2 which was able to utilise pyrene as sole source of carbon and energy was isolated from a polycyclic aromatic hydrocarbon (PAH) contaminated soil originating from the area of a former gaswork plant. The isolate metabolised up to 60 % of the pyrene added (0.5 mg/mL) within 8 days at 20 °C. Cis-4,5-pyrene dihydrodiol, 4,5-phenanthrene dicarboxylic acid, 1-hydroxy-2-naphthoic acid, 2-carboxybenzaldehyde, phthalic acid, and protocatechuic acid were identified as degradation products. Based on these findings a degradation pathway for pyrene is suggested which is in good accordance with the data published so far on bacterial pyrene metabolism.