Protein Separation and Hemocompatibility of Nitride Membranes in Microfluidic Filtration Systems.
Protein Separation and Hemocompatibility of Nitride Membranes in Microfluidic Filtration Systems.
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微流体过滤系统中氮化物膜的蛋白质分离和血液相容性。
DOI：
10.1109/embc.2018.8513538
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Johnson,DeanG
中科院分区：
文献类型：
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作者：
Salminen,Alec;Hill,Kayli;HenryChung,L;JamesMcGrath,L;Johnson,DeanG
改善接受血液透析（HD）的终末期肾病（ESRD）患者的健康结局需要可穿戴HD的新技术，例如可以在小设备占地面积内实现标准毒性清除率的高效膜。我们的小组已经开发出纳米多孔氮化硅（NPN）膜，比传统膜薄100至1000倍，透析效率高出几个数量级。进行逆流透析分离实验以测量尿素清除率，同时在搅拌烧杯中进行微透析实验以测量细胞色素-c和白蛋白的分离。进行血液透析实验，检测血小板活化和蛋白粘附。逆流实验的装置由聚二甲基硅氧烷（PDMS）和NPN膜芯片构成。逆流装置将尿素减少了20%之多。微透析实验显示，细胞色素-c的扩散约为60%，而白蛋白的清除率约为20%。初步血液相容性研究表明，与阳性对照（玻璃）相比，NPN膜表面不易发生蛋白质粘附和血小板活化。
Improving the health outcomes for end-stage renal Disease (ESRD) patients on hemodialysis (HD) requires new technologies for wearable HD such as a highly efficient membrane that can achieve standard toxic clearance rates in small device footprints. Our group has developed nanoporous silicon nitride (NPN) membranes which are 100 to 1000 times thinner than conventional membranes and are orders-ofmagnitude more efficient for dialysis. Counter flow dialysis separation experiments were performed to measure urea clearance while microdialysis experiments were performed in a stirred beaker to measure the separation of cytochrome-c and albumin. Hemodialysis experiments testing for platelet activation as well as protein adhesion were performed. Devices for the counter flow experiments were constructed with polydimethylsiloxane (PDMS) and a NPN membrane chip. The counter flow devices reduced the urea by as much as 20%. The microdialysis experiments showed a diffusion of ~ 60% for the cytochrome-c while clearing ~ 20% of the Albumin. Initial hemocompatibility studies show that the NPN membrane surface is less prone to both protein adhesion and platelet activation when compared to positive control (glass).