Human papillomavirus (HPV) E6/E7 mRNA detection in cervical exfoliated cells: a potential triage for HPV-positive women
Human papillomavirus (HPV) E6/E7 mRNA detection in cervical exfoliated cells: a potential triage for HPV-positive women
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DOI：
10.1631/jzus.b1600288
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发表时间：
2017-03-01
影响因子：
5.1
通讯作者：
Lu, Wei-guo
中科院分区：
文献类型：
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作者：
Yao, Ye-li;Tian, Qi-fang;Lu, Wei-guo
细胞学分诊通常被推荐给人类乳头瘤病毒(HPV)阳性的女性，但高度依赖训练有素的细胞学家。本研究旨在探讨宫颈脱落细胞中HPVE6/E7mRNA的检测是否可以作为临床人群中HPV阳性女性的潜在分类方法。对所有符合条件的HPV阳性妇女进行了初步HPV检测和巴氏杆菌(Pap)检测。采用QuantiVirus(R)HPVE6/E7mRNA检测宫颈脱落细胞中HPVE6/E7mRNA的表达。所有HPV阳性妇女都接受了阴道镜检查和进一步活检(如果有指征)。数据用皮尔逊卡方检验和受试者工作特性曲线进行评估。共有404名符合条件的HPV阳性妇女参加了登记。高度鳞状上皮内病变(HSIL)患者的E6/E7mRNA阳性率明显高于低度鳞状上皮内病变(LsIL)及正常组织。其敏感性(89.52%vs.86.67%，P=0.671)、特异性(48.96%vs.48.96%，P=1.000)、阳性预测值(39.00%vs.38.24%，P=1.000)和阴性预测值(92.76%vs.90.97%，P=0.678)无统计学差异。宫颈脱落细胞中HPVE6/E7mRNA的检测显示，对于HPV阳性的妇女，HPVE6/E7mRNA检测与巴氏分型在HSIL鉴定中的表现相同。HPVE6/E7mRNA检测可作为HPV阳性妇女的一种新的分诊选择。
Cytology triage has been generally recommended for human papillomavirus (HPV)-positive women, but is highly dependent on well-trained cytologists. The present study was designed to explore whether HPV E6/E7 mRNA detection in cervical exfoliated cells can be a potential triage for HPV-positive women from a clinic-based population. Both the primary HPV testing and Papanicolaou (Pap) test were performed on all eligible HPV-positive women. HPV E6/E7 mRNA was detected by QuantiVirus (R) HPV E6/E7 mRNA assay in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. The data were assessed by Pearson's Chi-squared test and the receiver operating characteristic curve. A total of 404 eligible HPV-positive women were enrolled. Positive rate of E6/E7 mRNA in high-grade squamous intraepithelial lesion (HSIL) cases was higher than that in low-grade squamous intraepithelial lesion (LSIL) or normal cases. There was no statistical difference found between mRNA and cytological testing with sensitivity (89.52% vs. 86.67%, P=0.671), specificity (48.96% vs. 48.96%, P=1.000), positive predictive value (39.00% vs. 38.24%, P=1.000), and negative predictive value (92.76% vs. 90.97%, P=0.678) for detecting >= HSIL. HPV E6/E7 mRNA detection in cervical exfoliated cells shows the same performance as Pap triage for HSIL identification for HPV-positive women. Detection of HPV E6/E7 mRNA may be used as a new triage option for HPV-positive women.