Quantitative expression of phospholipase C zeta, as an index to assess fertilization potential of a semen sample
Quantitative expression of phospholipase C zeta, as an index to assess fertilization potential of a semen sample
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DOI：
10.1093/humrep/der285
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发表时间：
2011-11-01
影响因子：
6.1
通讯作者：
Nasr-Esfahani, M. H.
中科院分区：
文献类型：
--
作者：
Aghajanpour, S.;Ghaedi, K.;Nasr-Esfahani, M. H.
背景：ICSI后受精失败主要归因于精子不能诱导卵母细胞活化。磷脂酶C ζ（PLC ζ）被认为是诱导卵母细胞活化的因素之一。本研究的目的是定量评估PLC zeta的表达在globozospermic男性或那些以前低或失败的受精与生育男性或那些具有高受精潜力。方法：采用Real-time PCR技术检测PLC zeta mRNA的相对表达量，并分析PLC zeta mRNA与其他精子标志物表达的相关性。结果：与正常生育男性（n = 24）相比，球形精子症男性（P < 0.01，n = 8）、低受精或受精失败者（P <0.01，n = 36）的PLC zeta表达明显降低。此外，与高受精个体（n = 17）相比，在球形精子症（P < 0.01）和先前低受精或失败的个体（P = 0.003）之间观察到显著差异。PLC zeta的表达与染色质成熟度或顶体酶活性无关。然而，受精率与PLC zeta的相对表达量之间存在显著相关性（r = 0.4，P < 0.01）。结论：本研究首次表明，PLC zeta的相对表达量的测定可能为ICSI后精子诱导卵母细胞活化的能力提供一个有用的指标。
BACKGROUND: Failed fertilization post-ICSI has been mainly attributed to the sperm's inability to induce oocyte activation. Phospholipase C zeta (PLC zeta) is considered to be one of the factors for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLC zeta in globozoospermic men or those with previously low or failed fertilization in comparison with fertile men or those with high fertilization potential. In addition, the relationship between expression of PLC zeta and that of other sperm markers was evaluated.METHODS: Real-time PCR was carried out to evaluate relative expression of PLC zeta mRNA. Chromatin maturity and acrosin activity were assessed by CMA3 staining and a colorimetric method.RESULTS: The expression of PLC zeta was significantly lower in globozoospermic men (P < 0.01, n = 8) or individuals with previously low or failed fertilization (P < 0.01, n = 36) in comparison to fertile men (n = 24). In addition, a significant difference was observed between globozoospermic (P < 0.01) and individuals with previously low or failed fertilization (P = 0.003) in comparison to high fertilization individuals (n = 17). Expression of PLC zeta was not correlated with either chromatin maturity or acrosin activity. However, a significant correlation was observed between the percentage of fertilization and relative expression of PLC zeta (r = 0.4, P < 0.01).CONCLUSION: In this study, for the first time, we have shown that assessment of relative expression of PLC zeta may provide a useful marker for the ability of sperm to induce oocyte activation after ICSI.