Construction and application of a zinc-specific biosensor for assessing the immobilization and bioavailability of zinc in different soils
Construction and application of a zinc-specific biosensor for assessing the immobilization and bioavailability of zinc in different soils
复制标题
锌特异性生物传感器的构建及应用，用于评估不同土壤中锌的固定化和生物利用度
DOI：
10.1016/j.envpol.2012.01.023
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发表时间：
2012-05-01
影响因子：
8.9
通讯作者：
Chen, Wenli
中科院分区：
文献类型：
--
作者：
Liu, Pulin;Huang, Qiaoyun;Chen, Wenli
利用lacZ基因融合技术研究了恶臭假单胞菌X4中不同czcRS操纵子的诱导性和特异性。β-糖苷酶活性的数据证实了czcR 3启动子对锌的定量响应。将无启动子的增强型绿色荧光蛋白（egfp）基因与恶臭假单胞菌（P. putida）X4染色体上的czcR 3启动子融合，构建了锌特异性生物传感器pczcR 3GFP。在四种不同的土壤与锌修正的水浸提液中，报告菌株检测到约90%的样品的锌含量。生物有效性评价和连续提取分析表明，锌的固定是高度依赖于土壤的理化性质。结果还表明，锌的不稳定性随着时间的推移而降低。它的结论是，生物传感器构成了一个替代系统，方便评价锌毒性在环境中。(C)2012爱思唯尔有限公司保留所有权利。
The inducibility and specificity of different czcRS operons in Pseudomonas putida X4 were studied by lacZ gene fusions. The data of beta-glycosidase activity confirmed that the czcR3 promoter responded quantitatively to zinc. A zinc-specific biosensor, P. putida X4 (pczcR3GFP), was constructed by fusing a promoterless enhanced green fluorescent protein (egfp) gene with the czcR3 promoter in the chromosome of P putida X4. In water extracts of four different soils amended with zinc, the reporter strain detected about 90% of the zinc content of the samples. Both the bioavailability assessment and the sequential extraction analysis demonstrated that the immobilization of zinc was highly dependent on the physicochemical properties of soils. The results also showed that the lability of zinc decreased over time. It is concluded that the biosensor constitutes an alternative system for the convenient evaluation of zinc toxicity in the environment. (C) 2012 Elsevier Ltd. All rights reserved.