Co-inoculation of human and murine carcinoma cells induces reciprocal suppression of metastasis by both cell lines.
Co-inoculation of human and murine carcinoma cells induces reciprocal suppression of metastasis by both cell lines.
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人类和小鼠癌细胞的共同接种诱导两种细胞系对转移的相互抑制。
DOI：
10.1023/a:1006607716061
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发表时间：
1999
影响因子：
4
通讯作者：
Testa,JE
中科院分区：
文献类型：
--
作者：
Nielsen-Preiss,SM;Quigley,JP;Testa,JE
用鸡胚胎转移实验研究了两个具有不同转移特性的细胞系之间的相互作用及其对扩散的影响。本实验对高侵袭性人表皮样细胞系Hep-3与小鼠结肠癌细胞系CL26进行了单独或混合实验。当单独接种时，每个细胞系都会在鸡胚中形成实验性转移，但只有Hep-3细胞会产生自发转移。在同时接种两种细胞系的胚胎中，在自发和实验转移试验中，转移负担总体上都减少了。进一步的研究表明，当CL26细胞与Hep-3细胞共同接种时，并没有获得自发转移的能力。然而，在CL26细胞存在的情况下，Hep-3的自发转移减少。静脉联合接种Hep-3和CL26细胞也能相互抑制这两种细胞系的实验性转移。这些研究表明，相邻的、表型不同的肿瘤细胞之间的相互作用可以对一种或两种细胞类型的扩散产生抑制作用。
The interactions of two cell lines having different metastatic properties, and the subsequent effects on dissemination were investigated using the chicken embryo metastasis assay. The highly aggressive human epidermoid cell line HEp-3 was tested alone or mixed with the mouse colon carcinoma cell line CL26 in this assay. When inoculated individually, each cell line forms experimental metastases in the chicken embryo, but only the HEp-3 cells give rise to spontaneous metastases. In embryos co-inoculated with both cell lines there was an overall reduction in metastatic burden in both the spontaneous and experimental metastasis assays. Furthers studies revealed that CL26 cells, when co-inoculated with HEp-3 cells did not acquire the ability to spontaneously metastasize. However, in the presence of CL26 cells, spontaneous HEp-3 metastasis was reduced. Intravenous co-inoculation of HEp-3 and CL26 cells also resulted in a reciprocal suppression of experimental metastasis by both cell lines. These studies demonstrate that the interactions of adjacent, phenotypically different tumor cells can have a suppressive effect on dissemination of one or both cell types.