Tensile Tests of Collagen Fibers Obtained from the Rabbit Patellar Tendon
Tensile Tests of Collagen Fibers Obtained from the Rabbit Patellar Tendon
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DOI：
10.1023/a:1009953805658
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发表时间：
1999-03-01
影响因子：
2.8
通讯作者：
Hayashi, Kozaburo
中科院分区：
文献类型：
--
作者：
Miyazaki, Hiroshi;Hayashi, Kozaburo
使用新开发的细胞和纤维性生物组织微拉伸测试系统，测定了直径约1 μ m的胶原纤维的拉伸性能。该测试系统由恒温试验箱、倒置显微镜、微操纵器、直接驱动线性执行器、悬臂式测压元件和视频尺寸分析仪（VDA）组成。用机械法从兔髌骨肌腱上切下的胶原束（直径约300 μ m）中分离纤维。使用氰基丙烯酸酯粘合剂将每根纤维的末端连接到一对玻璃微管（外径15至20 μ m）的尖端。其中一根微管连接在测压元件上；另一个与用于拉伸光纤的线性致动器相连。施加在纤维上的载荷是用测压元件测量的，而它的伸长是用VDA确定的，使用粘合剂边缘的图像作为标记。测试纤维的切线模量、抗拉强度和破坏应变分别为54.3 +/- 25.1 MPa、8.5 +/- 2:6 MPa和21.6 +/- 3:0%。这些数值与从兔髌骨肌腱上切下的胶原束（直径300 m m）和大块髌骨肌腱(Trans。J.生物医学。工程学报（英文版）；例如，纤维的抗拉强度和断裂应变分别约为纤维束的50%和200%。这些结果表明，纤维之间以及纤维与基质物质之间的力学相互作用对胶原束和大肌腱的力学性能有重要影响。
Tensile properties of collagen fibers of approximately 1 mu m in diameter were determined using a newly developed micro tensile test system for cells and fibrous biological tissues. The test system consists of a thermostatic test chamber, an inverted microscope, micromanipulators, a direct drive linear actuator, a cantilever-type load cell, and a video dimension analyzer (VDA). The fibers were isolated with a mechanical method from collagen fascicles (approximately 300 mu m in diameter) cut out from the rabbit patellar tendon. The ends of each fiber were attached to the tips of a pair of glass microtubes (15 to 20 mu m in outer diameter) using a cyanoacrylate adhesive. One of the microtubes was attached to the load cell; the other one was connected to the linear actuator which was utilized to stretch the fiber. Load applied to the fiber was measured with the load cell, while its elongation was determined with the VDA using the images of the edges of the adhesive as markers. Tangent modulus, tensile strength, and strain at failure of the tested fibers were 54.3 +/- 25: 1 MPa, 8.5 +/- 2: 6 MPa, and 21.6 +/- 3: 0%, respectively. These values were much different from those of collagen fascicles (300 m m in diameter) cut out from the rabbit patellar tendon and also from those of the bulk patellar tendon (Trans. ASME, J. Biomech. Eng. 121, 124-294, 1999); for example, tensile strength and strain at failure of the fibers were approximately 50 and 200% of those of the fascicles, respectively. These results suggest that the mechanical interactions between fibers and between fibers and ground substances contribute much to the mechanical properties of collagen fascicles and bulk tendons.