A RAPID METHOD OF VISUALIZING THE PANCREATIC-ISLETS FOR STUDIES OF ISLET CAPILLARY BLOOD-FLOW USING NONRADIOACTIVE MICROSPHERES
A RAPID METHOD OF VISUALIZING THE PANCREATIC-ISLETS FOR STUDIES OF ISLET CAPILLARY BLOOD-FLOW USING NONRADIOACTIVE MICROSPHERES
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DOI：
10.1111/j.1748-1716.1981.tb06909.x
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
HELLERSTROM, C
中科院分区：
文献类型：
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作者：
JANSSON, L;HELLERSTROM, C
描述了一种通过对冷冻和解冻的胰腺切片进行简单的暗场照射来显示大鼠胰岛的技术。通过这种方法，注射用于研究毛细血管血流的微球可以很容易地在胰岛和外分泌实质中定位和计数。该程序对于快速估计胰岛总数和研究其微循环都是有用的。
A technique is described for visualization of the pancreatic islets in the rat by simple dark field illumination of pieces of the frozen and thawed pancreas. By using this method, microspheres, injected for studies of the capillary blood flow, can be readily located and counted in both the islets and the exocrine parenchyma. The procedure could be useful both for rapid estimations of the total number of islets and for studies of their microcirculation.