Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes
Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes
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DOI：
10.1007/s00418-005-0014-z
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发表时间：
2005-08-01
影响因子：
2.3
通讯作者：
Roche, R
中科院分区：
文献类型：
--
作者：
Festy, F;Hoareau, L;Roche, R
脂肪组织含有一个易于分离的基质。因此，人脂肪组织提供了多能基质细胞的来源。为了确定脂肪细胞生物学中造血标志物的含义，我们定义了人脂肪组织来源的基质细胞的部分表型，并将其与完全分化的脂肪细胞进行比较。流式细胞术表明两种细胞类型的蛋白表达表型相似，包括CD10、CD13、CD34、CD36、CD55、CD59和CD65的表达。皮下和网膜脂肪组织之间没有显着差异，可以证明有关这些标志物的表达。然而，CD34、CD36和CD65的表达是细胞依赖性的。虽然基质细胞和成熟脂肪细胞之间的CD36和CD65的表达增加了一倍，但CD34的表达降低，尽管这种蛋白质存在于成熟脂肪细胞上。由于CD34被描述为干细胞标志物，并且它不太可能在分化的细胞上表达，因此通过免疫染色和蛋白质印迹证实了该结果。这种蛋白在脂肪细胞膜上的明确功能仍有待确定。成熟脂肪细胞上新蛋白质的表征可能对理解该组织的生物学具有广泛的意义。
Adipose tissue contains a stroma that can be easily isolated. Thus, human adipose tissue presents an source of multipotent stromal cells. In order to determine the implication of hematopoietic markers in adipocyte biology, we have defined part of the phenotype of the human adipose tissue-derived stromal cells, and compared this to fully differentiated adipocytes. Flow cytometry demonstrates that the protein expression phenotype of both cell types are similar and includes the expression of CD10, CD13, CD34, CD36, CD55, CD59 and CD65. No significant difference between subcutaneous and omental adipose tissue could be demonstrated concerning the expression of these markers. However, the expression of CD34, CD36 and CD65 is cell-dependent. While the expression of CD36 and CD65 doubled between stromal cells and mature adipocytes, the expression of CD34 decreased, despite this protein being present on the mature adipocyte. As CD34 is described as a stem cell marker and it being unlikely to be expressed on differentiated cells, this result was confirmed by immunostaining and western blot. The clear function of this protein on the adipocyte membrane remains to be determined. The characterization of new proteins on mature adipocytes could have broad implications for the comprehension of the biology of this tissue.