Metal Ion Binding to RNA
Metal Ion Binding to RNA
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DOI：
10.1039/978184973251200001
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Westhof, Eric
中科院分区：
文献类型：
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作者：
Auffinger, Pascal;Grover, Neena;Westhof, Eric
RNA晶体结构提供了大量关于与特定位点结合的局部金属离子的信息，例如RNA深沟、鸟嘌呤核苷酸的Hoogsteen面和阴离子磷酸氧。随着一些晶体结构被解决与重金属衍生物和其他“报告”离子，足够的信息可用于估计全球的相似性和差异的离子结合性能，并开始确定RNA和离子对彼此的影响。在这里，我们将讨论观察到的离子结合到RNA，它们的配位特性，以及它们在RNA结构研究中发挥的作用。对晶体学数据的分析强化了这样一个事实，即离子与核酸的相互作用不容易在相似电荷的离子之间互换。RNA-离子相互作用的生理相关性，主要涉及K+和Mg 2+阳离子，需要仔细分析，因为不同的结构是在非常不同的离子条件下解决的。分析是复杂的事实，分配并不总是准确的，往往在次优条件下进行，这进一步限制了这些离子可以建立的相互作用类型的泛化。
RNA crystal structures have provided a wealth of information on localized metal ions that are bound to specific sites, such as the RNA deep groove, the Hoogsteen face of guanine nucleotides and anionic phosphate oxygens. With a number of crystal structures being solved with heavy metal derivatives and other "reporter" ions, sufficient information is available to estimate global similarities and differences in ion binding properties and to begin determining the influence of RNA and ions on each other. Here we will discuss the ions that are observed bound to RNA, their coordination properties, and the roles they play in RNA structural studies. Analysis of the crystallographic data reinforces the fact that ion interactions with nucleic acids are not easily interchanged between similarly charged ions. The physiological relevance of RNA-ion interactions, mainly involving K+ and Mg2+ cations, needs to be analyzed with care as different structures are solved under very diverse ionic conditions. The analysis is complicated by the fact that the assignment is not always accurate, often done under sub-optimal conditions, which further limits the generalization about the types of interactions these ions can establish.