IgE antibodies against midge and moth found in Japanese asthmatic subjects and comparison of allergenicity between these insects
IgE antibodies against midge and moth found in Japanese asthmatic subjects and comparison of allergenicity between these insects
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DOI：
10.1111/j.1398-9995.1997.tb02548.x
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发表时间：
1997-01-01
期刊：
影响因子：
12.4
通讯作者：
Nakagawa, T
中科院分区：
文献类型：
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作者：
Komase, Y;Sakata, M;Nakagawa, T
用PharmaciaCAP系统检测了51例屋尘螨过敏性哮喘患者的蛾和摇蚊特异性IgE抗体。这些患者都没有明确的接触这些昆虫的历史或昆虫引起的哮喘症状的明显证据。进行RAST抑制试验以研究这两种昆虫之间的交叉致敏性。此外，IgE免疫印迹法进行了研究IgE结合成分的蛾和吸浆虫提取物。其中30例（59%）蛾类IgE抗体阳性，25例（49%）蠓类IgE抗体阳性。这些阳性频率与日本雪松花粉相似，日本雪松花粉众所周知会引起过敏。这两种昆虫的IgE抗体滴度之间存在显著的相关性（r=0.863）。RAST抑制试验的结果表明，这些昆虫之间的交叉过敏原，也存在物种特异性过敏原。蛾提取物中观察到15种IgE结合成分。最常见的IgE结合蛋白是79-kDa（84.2%），其次是72-kDa（78.9%），82-kDa（57.9%）和76-kDa（57.9%）蛋白。这些被认为是蛾类的主要过敏原。结果表明，蠓提取物中含有24种IgE结合组分。然而，没有观察到超过50%的患者血清反应的IgE结合蛋白。这些结果表明，这两种昆虫可能被认为是携带重要的过敏原，这两种昆虫之间存在交叉致敏性以及种特异性过敏原。
The specific IgE antibodies to moth (Bombyx mori) and midge (Chironomus yoshimatsui) were measured by the Pharmacia CAP system in 51 house-dust-mite-sensitive asthma patients. None of these patients had definite histories of exposure to these insects or apparent evidence of insect-induced asthma symptoms. The RAST-inhibition assay was performed to investigate cross-allergenicity between these two insects. Furthermore, IgE immunoblotting was done to study the IgE-binding components in moth and midge extracts. Thirty (59%) of these patients showed positive IgE antibodies to moth, while 25 (49%) showed positive IgE antibodies to midge. Those frequencies of positivity were similar to that for Japanese cedar pollen, which is well known to cause allergy. A significant correlation (r=0.863) was observed between IgE antibody titers of these two insects. The results from the RAST-inhibition assay indicated cross-allergenicity between these insects and also the existence of species-specific allergens. Fifteen IgE-binding components in moth extract were observed. The most frequent IgE-binding protein was the 79-kDa (84.2%), followed by the 72-kDa (78.9%), the 82-kDa (57.9%), and the 76-kDa (57.9%) proteins. Those were considered to be major allergens in moth. Twenty-four IgE-binding components in midge extract were observed. However, no IgE-binding protein to which over 50% of patient sera reacted was observed. These results suggest that these two insects may be considered to bear important allergens and that there is cross-allergenicity between these insects as well as species-specific allergens.