NEGATIVE-ION FAST-ATOM-BOMBARDMENT TANDEM MASS-SPECTROMETRY TO DETERMINE SULFATE AND LINKAGE POSITION IN GLYCOSAMINOGLYCAN-DERIVED DISACCHARIDES
NEGATIVE-ION FAST-ATOM-BOMBARDMENT TANDEM MASS-SPECTROMETRY TO DETERMINE SULFATE AND LINKAGE POSITION IN GLYCOSAMINOGLYCAN-DERIVED DISACCHARIDES
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DOI：
10.1016/1044-0305(92)80002-3
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发表时间：
1992-11-01
影响因子：
3.2
通讯作者：
LINHARDT, RJ
中科院分区：
文献类型：
--
作者：
LAMB, DJ;WANG, HM;LINHARDT, RJ
负离子快原子轰击串联质谱法已被用于单硫酸化二糖的分析。这些商业上获得的二糖已经使用多糖裂解酶从糖胺聚糖酶促制备。分析了来自硫酸软骨素和硫酸皮肤素的三种二糖和来自硫酸乙酰肝素和化学衍生肝素的两种二糖。所有五种二糖都是异构体，硫酸酯位置和连接位置不同。当硫酸基团位于不同的糖单元上时，全扫描质谱可用于区分异构体。这种结构信息是从通过糖苷键裂解产生的碎片离子中获得的。每种单硫酸化二糖的全扫描质谱也产生具有长寿命的强分子阴离子。碰撞激活这些导致串联质谱丰富的显着产品离子。这些碎片离子中的一些是通过环断裂形成的，并且在硫酸根和键合位置的测定中是有用的。
Negative ion fast-atom bombardment tandem mass spectrometry has been used in the analysis of monosulfated disaccharides. These commercially obtained disaccharides have been enzymatically prepared from glycosaminoglycans using polysaccharide lyases. Three disaccharides from chondroitin sulfate and dermatan sulfate and two disaccharides from heparan sulfate and chemically derivatized heparin were analyzed. All five disaccharides were isomeric, with differences in sulfate position and linkage position. The full-scan mass spectra are useful in differentiating isomers when the sulfate group resides on different saccharide units. This structural information was obtained from fragment ions produced through cleavage at the glycosidic linkage. The full-scan mass spectra of each monosulfated disaccharide also produced intense molecular anions having long lifetimes. Collisional activation of these resulted in tandem mass spectra rich in significant product ions. Some of these fragment ions were formed through ring cleavage and were useful in the determination of both sulfate and linkage position.