Tryptophan biosynthesis in Nicotiana tabacum and Daucus carota cell cultures: site of action of inhibitory tryptophan analogs.
Tryptophan biosynthesis in Nicotiana tabacum and Daucus carota cell cultures: site of action of inhibitory tryptophan analogs.
复制标题
烟草和胡萝卜细胞培养物中的色氨酸生物合成：抑制性色氨酸类似物的作用位点。
DOI：
10.1016/0304-4165(72)90311-x
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
J. Widholm
中科院分区：
文献类型：
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作者：
J. Widholm
1.1.8个色氨酸类似物完全抑制烟草和胡萝卜细胞的生长，其中5-甲基色氨酸、4-甲基色氨酸、5-氟色氨酸和6-氟色氨酸的抑制作用最强，5-羟基色氨酸、3-β-吲哚丙烯酸、7-氮杂环丙氨酸和3-甲基邻氨基苯甲酸的抑制作用较差。除3-β-吲哚和3-甲基邻氨基苯甲酸外，其余类似物的生长抑制作用均可被邻氨基苯甲酸、吲哚或色氨酸逆转。邻氨基苯甲酸合成酶，通常是反馈抑制的Byl-色氨酸，比色氨酸合成酶对所有类似物的抑制更敏感。结果表明，除3-β-吲哚丙烯酸和3-甲基邻氨基苯甲酸外，其余类似物均通过抑制邻氨基苯甲酸合成酶有效地阻止色氨酸的生物合成而发挥作用。色氨酸的缺乏阻碍了细胞的生长。
1.1. Eight tryptophan analogs completely inhibit the growth ofNicotiana tabacum(tobacco) andDaucus carota(carrot) cells with 5-methyltryptophan, 4-methyltryptophan, 5-fluorotrytophan and 6-fluorotryptophan being the most effective with 5-hydroxytryptophan, 3β-indoleacrylic acid, 7-azatryptophan, and 3-methylanthranilic acid less effective.2.2. The growth inhibition caused by all the analogs, except 3β-indoleacrylic acid and 3-methylanthranilic acid, could be reversed by the addition to the medium of anthranilic acid, indole orl-tryptophan.3.3. Anthranilate synthetase, which is normally feedback inhibited byl-tryptophan, was much more sensitive to inhibition by all the analogs than was tryptophan synthetase.4.4. The evidence indicates that all of the analogs, except 3β-indoleacrylic acid and 3-methylanthranilic acid, act by inhibiting anthranilate synthetase which effectively stops the biosynthesis of tryptophan. The lack of tryptophan prevents cell growth.