Flowering of Arabidopsis cop1 mutants in darkness
Flowering of Arabidopsis cop1 mutants in darkness
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DOI：
10.1093/pcp/pch047
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发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Komeda, Y
中科院分区：
文献类型：
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作者：
Nakagawa, M;Komeda, Y
为了阐明COP]基因在开花中的作用，我们分析了COP]突变系在黑暗中的开花情况。当在1% （w/v）蔗糖的环境中生长时，cop1-6突变体在黑暗中开花，而cop1-1和cop1-4则没有开花。然而，cop1-1和cop1-4在5% （w/v）蔗糖的存在下在黑暗中开花。因此，COP]基因不仅抑制幼苗的光形态发生，也抑制黑暗中的开花。通过比较cop1-6和野生型植物在黑暗环境中的花特性基因mRNA水平发现，cop1-6中CO下游基因mRNA水平升高，FLC mRNA水平降低。cop1-6的双突变体和cry2-1、gi-2、co-1和ld-1的晚花突变体在黑暗中开花。除cry2-1外，所有双突变体cop1-6的开花时间都晚于cop1-6，表明cop1-6对cry2-1具有上位性。ld-1 cop1-6双突变体的开花时间比ld-1突变体早得多。双突变体的开花延迟不受光照条件的强烈影响，而gi-2 cop1-6双突变体的开花延迟在黑暗条件下有所减少。
To elucidate the role of the COP] gene in flowering, we analyzed flowering of cop] mutant lines in darkness. When grown in the presence of 1% (w/v) sucrose, the cop1-6 mutant flowered in darkness, but cop1-1 and cop1-4 did not. However, cop1-1 and cop1-4 flowered in darkness when grown in the presence of 5% (w/v) sucrose. Therefore, the COP] gene represses not only photomorphogenesis in seedlings but also flowering in darkness. Comparison of mRNAs levels of floral identity genes in cop1-6 and wildtype plants grown in darkness revealed increased mRNA levels of genes that act downstream of CO and reduced FLC mRNA level in cop1-6. Double mutants of cop1-6 and each of the late-flowering mutations cry2-1, gi-2, co-1, and ld-1 flowered in darkness. All of the double mutants except cry2-1 cop1-6 flowered later than cop1-6, demonstrating that cop1-6 is epistatic to cry2-1 for early flowering. The ld-1 cop1-6 double mutant flowered much earlier than the ld-1 mutant. The delay in flowering in the double mutants was not strongly influenced by the light conditions, whereas that of the gi-2 cop1-6 double mutant was reduced in darkness.