Glucose homeostasis in the nonobese diabetic mouse at the prediabetic stage
Glucose homeostasis in the nonobese diabetic mouse at the prediabetic stage
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DOI：
10.1210/en.139.3.1115
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发表时间：
1998-03-01
期刊：
影响因子：
4.8
通讯作者：
Homo-Delarche, F
中科院分区：
文献类型：
--
作者：
Amrani, A;Durant, S;Homo-Delarche, F
由于在非肥胖糖尿病（NOD）小鼠的早期糖尿病前期阶段的葡萄糖稳态的数据很少，我们研究了基础条件下，4，6和8周龄喂养的NOD小鼠的性别，胰岛素血症和胰腺胰岛素含量，与性别和年龄匹配的喂养C57 BL/6小鼠相比。我们还研究了禁食8周龄NOD和C57 BL/6小鼠的两种性别的葡萄糖耐量。在基础喂养条件下获得的主要结果，当比较两种菌株时，在所有年龄段的NOD女性和NOD男性（特别是在6周龄时）中，NOD女性的胰岛素水平较低，胰岛素血症较高。葡萄糖耐量试验表明：1）8周龄NOD小鼠对1g/kg ip葡萄糖的血糖反应持续时间较对照组短; 2）8周龄NOD小鼠对葡萄糖的血胰岛素反应持续时间较对照组短; 3）8周龄NOD小鼠对葡萄糖的血胰岛素反应持续时间较对照组短。（1 g/kg ip）在两种性别NOD小鼠中均早于Iri性别匹配的C57 BL/6小鼠; 3）NOD小鼠与对照组相比，存在一种不寻常的两性异形现象，雌性小鼠对葡萄糖的反应是雄性小鼠分泌胰岛素的两倍; 4）剂量反应研究（1-6 g/kg葡萄糖）证实了NOD小鼠的两种性别中血糖水平的较低增加以及它们在胰岛素分泌方面的不寻常的性二态性;和5）在4- 8周龄NOD小鼠中的葡萄糖耐量试验表明，虽然在4周龄小鼠中未观察到胰岛素分泌的两性异形，但在6周龄时尤其明显。综上所述，这些结果表明，p-细胞过度活跃存在于NOD小鼠在早期糖尿病前期阶段，特别是在NOD女性。
Because few data were available on glucose homeostasis at the early prediabetic stage in the nonobese diabetic (NOD) mouse, we investigated glycemia, insulinemia, and pancreatic insulin content under basal conditions in both sexes of 4-, 6-, and 8-week-old fed NOD mice, compared with sex-and age-matched fed C57BL/6 mice. We also investigated glucose tolerance in both sexes of fasting 8-week-old NOD and C57BL/6 mice. The main results obtained under basal fed conditions, when comparing both strains, were lower glycemia and higher insulinemia in NOD females at all ages investigated and in NOD males (particularly at 6 weeks of age). Glucose tolerance tests showed that: 1) the blood glucose response to 1 g/kg ip glucose was less sustained in both sexes of 8-week-old NOD mice than in their control counterparts; 2) the blood insulin response to glucose (1 g/kg ip) appeared earlier in both sexes of NOD mice than iri sex-matched C57BL/6 mice; 3) an unusual sexual dimorphism existed in NOD mice, compared with controls, with females secreting, in response to glucose, twice as much insulin as males; 4) dose-response studies (1-6 g/kg glucose) confirmed the lower increase in blood glucose levels in both sexes of NOD mice and their unusual sexual dimorphism in insulin secretion; and 5) glucose tolerance tests in 4- to 8-week-old NOD mice showed that although the sexual dimorphism in insulin secretion was not observed in 4-week-old mice, it was particularly striking at 6 weeks of age. Taken together, these results suggest that p-cell hyperactivity exists in the NOD mouse at the early prediabetic stage, especially in NOD females.