Involvement of a plasmid in growth on and dispersion of crude oil by Acinetobacter calcoaceticus RA57
Involvement of a plasmid in growth on and dispersion of crude oil by Acinetobacter calcoaceticus RA57
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质粒参与乙酸钙不动杆菌 RA57 在原油上的生长和分散
DOI：
10.1128/aem.53.8.1918-1923.1987
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发表时间：
1987
影响因子：
4.4
通讯作者：
D. Gutnick
中科院分区：
文献类型：
--
作者：
Sandra Rusansky;R. Avigad;S. Michaeli;D. Gutnick
采用标准富集培养技术分离出一种原油降解不动杆菌——钙酸不动杆菌RA57，其利用当地加油站附近含油污泥的能力。菌株RA57含有4个质粒：pSR1 （5.1 kb）、pSR2 （5.4 kb）、pSR3 （10.5 kb）和pSR4 （20 kb）。通过双向凝胶电泳鉴定了前三个质粒的超螺旋和开圆形形态。pSR4的内切酶分析表明，该质粒含有一个圆形图。在吖啶橙存在下随机分离菌落，发现菌落分为两类：(i)在液体培养中失去对原油的生长和分散能力并同时被pSR4固化的菌落；（ii）保留对原油的生长和分散能力并含有pSR4的菌落。第一类菌株在烃类蒸气中继续生长，这表明pSR4固化过程中的缺陷与细胞与烃类底物的物理相互作用有关，而不是与其代谢有关。两类突变体在对碳氢化合物的粘附性或细胞外乳化活性的产生方面没有差异。在含pSR4和不含pSR4的菌株对原油混合培养的生长试验中，没有发现生长缺陷。这些结果与pSR4编码一个与细胞表面紧密相关的因子的可能性一致。
A crude-oil-degrading Acinetobacter species, Acinetobacter calcoaceticus RA57, was isolated by standard enrichment culture techniques on the basis of its ability to utilize the oily sludge found in the vicinity of a local gas station. Strain RA57 was found to contain four plasmids: pSR1 (5.1 kilobases [kb]), pSR2 (5.4 kb), pSR3 (10.5 kb), and pSR4 (20 kb). Both supercoiled and open circular forms of the first three plasmids were identified by two-dimensional gel electrophoresis. Restriction endonuclease analysis of pSR4 demonstrated that the plasmid contained a circular map. Colonies were isolated at random after growth in the presence of acridine orange and found to fall into two categories: (i) those which had lost the ability to grow on and disperse crude oil in liquid culture and concurrently were cured of pSR4 and (ii) those which retained the ability to both grow on and disperse crude oil and which contained pSR4. Strains from the first class continued to grow on hydrocarbon vapors, indicating that the defect associated with the curing of pSR4 was related to the physical interaction of the cells with the hydrocarbon substrate, rather than to its metabolism. No differences in either adherence to hydrocarbons or production of extracellular emulsifying activity were found between the two classes of mutants. In growth experiments on crude oil in mixed culture with strains which either contained or lacked pSR4, no sparing of the growth defect was observed. The results are consistent with the possibility that pSR4 encodes a factor(s) which is tightly associated with the cell surface.