EXPRESSION OF THE NUCLEAR GENE ENCODING OXYGEN-EVOLVING ENHANCER PROTEIN-2 IS REQUIRED FOR HIGH-LEVELS OF PHOTOSYNTHETIC OXYGEN EVOLUTION IN CHLAMYDOMONAS-REINHARDTII
EXPRESSION OF THE NUCLEAR GENE ENCODING OXYGEN-EVOLVING ENHANCER PROTEIN-2 IS REQUIRED FOR HIGH-LEVELS OF PHOTOSYNTHETIC OXYGEN EVOLUTION IN CHLAMYDOMONAS-REINHARDTII
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DOI：
10.1073/pnas.84.3.749
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发表时间：
1987-02-01
影响因子：
11.1
通讯作者：
ROCHAIX, JD
中科院分区：
文献类型：
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作者：
MAYFIELD, SP;RAHIRE, M;ROCHAIX, JD
我们已经克隆了一个cDNA编码的20 kDa的多肽，氧进化增强蛋白2（OEE 2），在莱茵衣藻。这种多肽与光合放氧有关，它与光系统II复合体有关，光系统II复合体是所有高等植物和藻类中放氧的位点。给出了OEE 2 cDNA序列、前蛋白的氨基酸序列、成熟OEE 2蛋白的N-末端蛋白序列以及OEE 2基因的编码区。该蛋白质由57个氨基酸的N-末端转运肽合成，该肽指导该多肽穿过三个细胞膜的转运。C.缺乏放氧活性的莱茵衣藻显示出特异性缺失OEE 2多肽。该突变导致所有OEE 2 mRNA和蛋白质完全缺失，不影响其他光系统II多肽或其mRNA的积累。
We have cloned a cDNA encoding a 20-kDa polypeptide, oxygen-evolving enhancer protein 2 (OEE2), in Chlamydomonas reinhardtii. This polypeptide has been implicated in photosynthetic oxygen evolution, and it is associated with the photosystem II complex, the site of oxygen evolution in all higher plants and algae. The sequence of OEE2 cDNA, the deduced amino acid sequence of the preprotein, the N-terminal protein sequence of mature OEE2 protein, and the coding regions of the single OEE2 gene are presented. The protein is synthesized with a 57-amino acid N-terminal transit peptide that directs the transport of this polypeptide across three cellular membranes. A nuclear mutant of C. reinhardtii, deficient in oxygen-evolving activity, is shown to be specifically missing OEE2 polypeptides. This mutation, which results in the complete absence of all OEE2 mRNA and protein, does not affect the accumulation of other photosystem II polypeptides or their mRNAs.