Early diagnosis of hepatocellular carcinoma with lectin electrophoresis of serum alpha-fetoprotein.
Early diagnosis of hepatocellular carcinoma with lectin electrophoresis of serum alpha-fetoprotein.
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血清甲胎蛋白凝集素电泳对肝细胞癌的早期诊断。
DOI：
10.1159/000217551
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
H. Kaneda
中科院分区：
文献类型：
--
作者：
H. Taga;H. Hirai;H. Ishizuka;H. Kaneda
建立了一种灵敏的甲胎蛋白（AFP）凝集素亲和电泳方法。将凝集素凝胶吸附的AFP印迹于预先包被AFP特异性抗体的硝酸纤维素膜上，用过氧化物酶标记的抗AFP抗体染色。这种方法可以检测到低至4微克/升的纯化AFP溶解在缓冲液中，或50微克/升的血清标本。许多肝病患者在东京日本大学医院接受了长期随访。连续采集血清标本并冷冻保存。我们重新回顾性研究了6系列血清标本的凝集素免疫印迹技术，发现3例显示肝细胞癌特异性AFP变异在非常早期的阶段，在任何其他证据的肝细胞癌的临床检查。
A sensitive procedure involving lectin affinity electrophoresis of alpha-fetoprotein (AFP) was established. AFPs electrophoresed on lectin-containing gels were blotted on nitrocellulose membrane which was precoated with the specific antibody to AFP and stained with peroxidase-labeled anti-AFP antibody. This method could detect as little as 4 micrograms/l of purified AFP dissolved in buffer, or 50 micrograms/l in serum specimens. A number of patients with liver disease have been followed for long periods in Nihon University Hospital, Tokyo. Serum specimens were collected serially and stored frozen. We have reinvestigated retrospectively 6 series of serum specimens by the lectin-immunoblotting technique and found 3 cases that revealed a hepatocellular carcinoma-specific AFP variant at a very early stage, in advance of any other evidence of hepatocellular carcinoma by clinical examination.