Loss of ability to synthesize collagen in fibroblasts transformed by rous sarcoma virus.
Loss of ability to synthesize collagen in fibroblasts transformed by rous sarcoma virus.
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劳斯肉瘤病毒转化的成纤维细胞丧失合成胶原蛋白的能力。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
T. Z. Liu
中科院分区：
文献类型：
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作者：
W. Levinson;R. Bhatnagar;T. Z. Liu
Rous肉瘤病毒对鸡胚成纤维细胞的转化抑制了其合成胶原的能力。动力学实验表明，感染后72小时，胶原合成减少90%。非转化性ROU相关病毒不抑制胶原合成。这种抑制是由于细胞合成胶原多肽的失败，而不是由于Pro羟基酶活性的降低；Pro羟基酶的水平是未感染细胞的四倍。单独或同时加入二丁酰环AMP和茶碱并不能恢复胶原的合成。
Transformation of chick embryo fibroblasts by Rous sarcoma virus inhibited their ability to synthesize collagen. Kinetic experiments showed that 72 hours after infection, collagen synthesis was reduced by 90%. Nontransforming Rous-associated viruses did not inhibit collagen synthesis. The inhibition resulted from the failure of the cells to synthesize collagen polypeptides rather than from a decrease in the activity of prolyl hydroxylase; the levels of prolyl hydroxylase were fourfold those in uninfected cells. The addition of dibutyryl cyclic AMP and theophylline, alone or together, did not restore collagen synthesis.