Assessment of Perfused Foveal Microvascular Density and Identification of Nonperfused Capillaries in Healthy and Vasculopathic Eyes
Assessment of Perfused Foveal Microvascular Density and Identification of Nonperfused Capillaries in Healthy and Vasculopathic Eyes
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DOI：
10.1167/iovs.14-15136
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发表时间：
2014-12-01
影响因子：
4.4
通讯作者：
Rosen, Richard B.
中科院分区：
文献类型：
--
作者：
Pinhas, Alexander;Razeen, Moataz;Rosen, Richard B.
目的.采用自适应光学扫描光检眼镜荧光血管造影（AOSLO FA）技术，对健康青年眼和老年血管病变眼的黄斑中心凹微血管进行分析。使用同时共焦红外（IR）反射（790 nm）和荧光（488 nm）通道，对中心凹中心800 μ m半径范围内的表层视网膜微血管进行AOSLO FA成像。相应的IR结构和FA灌注图进行了相互比较，以确定邻近中心凹无血管区的非灌注毛细血管。微血管密度计算从荧光FA灌注图。对16名健康成人（26只眼;平均年龄25岁，范围21-29）和6名视网膜血管病变患者（6只眼;平均年龄55岁，范围44-70）进行成像。至少有一个非灌注毛细血管中观察到的16个健康的nonfellow眼睛和4个6血管病变的眼睛。与健康眼相比，血管病变眼的毛细血管无灌注更广泛。16只健康非对侧眼的微血管密度为42.0 ± 4.2 mm（-1）（范围33-50 mm（-1））。所有6只血管病变眼的微血管密度均降低。AOSLO FA提供了一种估计中心凹微血管密度的体内方法，并揭示了隐匿性非灌注视网膜毛细血管。在健康的年轻人中，无灌注毛细血管可能代表正常的变异和/或病理学的早期迹象。虽然有限，这里提出的规范性数据是一个步骤，发展临床有用的微血管参数的眼睛和/或全身性疾病。
PURPOSE. To analyze the foveal microvasculature of young healthy eyes and older vasculopathic eyes, imaged using in vivo adaptive optics scanning light ophthalmoscope fluorescein angiography (AOSLO FA).METHODS. AOSLO FA imaging of the superficial retinal microvasculature within an 800-mu m radius from the foveal center was performed using simultaneous confocal infrared (IR) reflectance (790 nm) and fluorescence (488 nm) channels. Corresponding IR structural and FA perfusion maps were compared with each other to identify nonperfused capillaries adjacent to the foveal avascular zone. Microvascular densities were calculated from skeletonized FA perfusion maps.RESULTS. Sixteen healthy adults (26 eyes; mean age 25 years, range, 21-29) and six patients with a retinal vasculopathy (six eyes; mean age 55 years, range, 44-70) were imaged. At least one nonperfused capillary was observed in five of the 16 healthy nonfellow eyes and in four of the six vasculopathic eyes. Compared with healthy eyes, capillary nonperfusion in the vasculopathic eyes was more extensive. Microvascular density of the 16 healthy nonfellow eyes was 42.0 +/- 4.2 mm(-1) (range, 33-50 mm(-1)). All six vasculopathic eyes had decreased microvascular densities.CONCLUSIONS. AOSLO FA provides an in vivo method for estimating foveal microvascular density and reveals occult nonperfused retinal capillaries. Nonperfused capillaries in healthy young adults may represent a normal variation and/or an early sign of pathology. Although limited, the normative data presented here is a step toward developing clinically useful microvascular parameters for ocular and/or systemic diseases.