Effect of antiplaque compounds and mouthrinses on the activity of glucosyltransferases from Streptococcus sobrinus and insoluble glucan production
Effect of antiplaque compounds and mouthrinses on the activity of glucosyltransferases from Streptococcus sobrinus and insoluble glucan production
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DOI：
10.1111/j.1399-302x.2008.00441.x
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发表时间：
2008-10-01
影响因子：
--
通讯作者：
Badet, C.
中科院分区：
文献类型：
--
作者：
Furiga, A.;Dols-Lafargue, M.;Badet, C.
简介：开发抑制葡糖基转移酶（GTF）活性及其产生葡聚糖的治疗剂是减少龋齿的潜在策略。本研究的目的是第一次表征GTF制剂远缘链球菌ATCC 33478，然后选择化合物和漱口水对不溶性葡聚糖（ISG）的形成相结合的GTFs.Methods的影响进行评估：通过电泳的粗GTF混合物的纯度。分析了pH、温度、蔗糖和葡聚糖T10浓度对GTF活性的影响，并对产物的化学结构进行了研究。最后，通过总反应速率和ISG合成量的降低，分析了用于口腔卫生的市售漱口水及其活性成分（氯己定、多酚化合物、氟化物衍生物、多元醇、氯化十六烷基吡啶和聚维酮碘）对GTF的抑制作用。获得的sobrinus ATCC 33478粗GTF制剂含有该物种已知的四种不同GTF的混合物。对于最佳粘附ISG形成，反应参数为37 ℃，pH 6.5，蔗糖50 g/l，和葡聚糖T10 2 g/l。在这些条件下，最有效的药物是氯己定，氯化十六烷基吡啶和单宁酸。Eludril（R）、Elmex（R）和Betadine（R）是所有测试的漱口水中最有效的抑制剂。结论：由于商业产品的配方相当大地影响活性成分的效率，因此本研究中开发的快速代表性ISG抑制测试应该是非常有趣的。
Introduction: The development of therapeutic agents inhibiting the activity of glucosyltransferases (GTF) and their production of glucans is a potential strategy to reduce dental decay. The aim of this study was first to characterize a GTF preparation from Streptococcus sobrinus ATCC 33478 and then to evaluate the effects of select compounds and mouthrinses on insoluble glucan (ISG) formation by combined GTFs.Methods: The purity of the crude GTF mixture was assessed by electrophoresis. The effects of pH, temperature, sucrose, and dextran T10 concentrations on GTF activity were analyzed and the chemical structure of the products was investigated. Finally, the inhibition of GTF by commercial mouthrinses used in oral hygiene and their active components (chlorhexidine, polyphenolic compounds, fluoride derivatives, polyols, cetylpyridinium chloride, and povidone iodine) was analyzed through the reductions in the overall reaction rate and the quantity of ISG synthesized.Results: The S. sobrinus ATCC 33478 crude GTF preparation obtained contains a mixture of four different GTFs known for this species. For optimal adherent ISG formation, the reaction parameters were 37 degrees C, pH 6.5, sucrose 50 g/l, and dextran T10 2 g/l. Under these conditions, the most effective agents were chlorhexidine, cetylpyridinium chloride, and tannic acid. Eludril (R), Elmex (R), and Betadine (R) were the most effective inhibitors of all the mouthrinses tested.Conclusion: As the formulation of commercial products considerably influences the efficiency of active components, the fast representative ISG inhibition test developed in this study should be of great interest.