The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development.
The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development.
复制标题
DOI：
10.1083/jcb.201202135
复制
发表时间：
2012-08-06
期刊：
影响因子：
--
通讯作者：
Hiiragi T
中科院分区：
文献类型：
--
作者：
Courtois A;Schuh M;Ellenberg J;Hiiragi T
从减数分裂样纺锤体形成的特点是缺乏中心粒的有丝分裂纺锤体的过渡逐渐发生在胚胎植入前阶段。从减数分裂到有丝分裂的转变，经典上定义为受精，是发育的一个基本过程。然而，其机制在很大程度上仍未被探索。在本文中，我们报告了一个令人惊讶的逐渐过渡，从减数分裂到有丝分裂的前八个分裂的小鼠胚胎。第一次分裂仍然在很大程度上共享纺锤体形成的机制与减数分裂，在此期间，纺锤体是自组装从随机分布的微管组织中心（MTOCs）没有中心粒，因为动力蛋白和驱动蛋白-5的协同活动。在着床前发育过程中，细胞MTOC的数量逐渐减少，纺锤体极点逐渐变得更加集中，纺锤体长度随着细胞大小逐渐缩小。典型的有丝分裂纺锤体仅在囊胚中建立，其中中心蛋白、odf 2、驱动蛋白-12和CP 110阳性中心体。总体而言，从减数分裂到有丝分裂的转变在小鼠胚胎的整个植入前阶段逐渐进行，从而提供了一个独特的系统来研究体内中心体生物发生的机制。
The transition from a meiotic-like spindle formation characterized by lack of centrioles to a typical mitotic spindle occurs gradually in embryos during the preimplantation stage. The transition from meiosis to mitosis, classically defined by fertilization, is a fundamental process in development. However, its mechanism remains largely unexplored. In this paper, we report a surprising gradual transition from meiosis to mitosis over the first eight divisions of the mouse embryo. The first cleavages still largely share the mechanism of spindle formation with meiosis, during which the spindle is self-assembled from randomly distributed microtubule-organizing centers (MTOCs) without centrioles, because of the concerted activity of dynein and kinesin-5. During preimplantation development, the number of cellular MTOCs progressively decreased, the spindle pole gradually became more focused, and spindle length progressively scaled down with cell size. The typical mitotic spindle with centrin-, odf2-, kinesin-12–, and CP110-positive centrosomes was established only in the blastocyst. Overall, the transition from meiosis to mitosis progresses gradually throughout the preimplantation stage in the mouse embryo, thus providing a unique system to study the mechanism of centrosome biogenesis in vivo.
登录
查看更多内容
影响因子：
9.2
作者：
Hyman, AA
通讯作者：
Hyman, AA
影响因子：
5.8
作者：
Baddeley, A;Turner, R
通讯作者：
Turner, R
影响因子：
64.8
作者：
Carazo-Salas, RE;Guarguaglini, G;Mattaj, IW
通讯作者：
Mattaj, IW
DOI：
10.1523/jneurosci.3739-10.2010
发表时间：
2010-11-03
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Liu M;Nadar VC;Kozielski F;Kozlowska M;Yu W;Baas PW
通讯作者：
Baas PW
影响因子：
7.8
作者：
Lane, HA;Nigg, EA
通讯作者：
Nigg, EA