Identity of human Lyb-2 and CD72 and localization of the gene to chromosome 9.
Identity of human Lyb-2 and CD72 and localization of the gene to chromosome 9.
复制标题
人类 Lyb-2 和 CD72 的同一性以及该基因在 9 号染色体上的定位。
DOI：
10.1002/eji.1830210615
复制
发表时间：
1991
影响因子：
5.4
通讯作者：
Parnes,JR
中科院分区：
文献类型：
--
作者：
VonHoegen,I;Hsieh,CL;Scharting,R;Francke,U;Parnes,JR
我们最近报道了编码小鼠B细胞分化抗原LyB-2的人同源物的cDNA克隆的分离。LyB-2的表达仅限于B谱系细胞，并在小鼠和人的抗体分泌浆细胞中关闭。抗小鼠LyB-2单克隆抗体（mAb）的功能研究表明，该蛋白可能参与B细胞增殖的信号。我们现在描述人Lyb-2特异性mAb的产生。此外，我们证明了人LyB-2蛋白被mAb识别，该mAb对新聚集的泛B细胞表面抗原CD 72具有特异性，该抗原已由mAb定义。表达编码人Lyb-2的转染cDNA的小鼠L（tk−）细胞结合CD 72特异性mAb。我们进一步表明，抗血清和人LyB-2特异性mAb以及抗CD 72 mAb可免疫沉淀来自人脾B细胞和B细胞系以及转染的L（tk−）细胞的相同蛋白。这些数据表明，CD 72是小鼠Lyb-2的人类等效物。我们还将人类Lyb-2/CD 72基因定位在9号染色体的短臂上。小鼠Lyb-2先前已被定位于小鼠4号染色体。Lyb-2/CD 72是目前已知位于人类9号染色体和小鼠4号染色体上的第十个基因。
We have recently reported the isolation of cDNA clones encoding the human homolog of the mouse B cell differentiation antigen Lyb‐2. Expression of Lyb‐2 is restricted to B lineage cells and turned off in antibody‐secreting plasma cells in both mice and humans. Functional studies with anti‐mouse Lyb‐2 monoclonal antibodies (mAb) suggest that this protein may be involved in signals for B cell proliferation. We now describe the generation of mAb specific for human Lyb‐2. Furthermore, we demonstrate that the human Lyb‐2 protein is recognized by a mAb specific for the newly clustered pan B cell surface antigen CD72, which had been defined by mAb. Mouse L(tk−) cells expressing a transfected cDNA encoding human Lyb‐2 bind a mAb specific for CD72. We further show that an antiserum and a mAb specific for human Lyb‐2 and an anti‐CD72 mAb immunoprecipitate the identical protein from human splenic B cells and B cell lines, as well as from transfected L(tk−) cells. These data indicate that CD72 is the human equivalent of mouse Lyb‐2. We have additionally localized the gene for human Lyb‐2/CD72 on the short arm of chromosome 9. Mouse Lyb‐2 had been previously mapped to mouse chromosome 4. Lyb‐2/CD72 is now the tenth gene known to be localized on human chromosome 9 and mouse chromosome 4.