EXPRESSION OF A NEURALLY RELATED LAMININ BINDING-PROTEIN BY NEURAL CREST-DERIVED CELLS THAT COLONIZE THE GUT - RELATIONSHIP TO THE FORMATION OF ENTERIC GANGLIA
EXPRESSION OF A NEURALLY RELATED LAMININ BINDING-PROTEIN BY NEURAL CREST-DERIVED CELLS THAT COLONIZE THE GUT - RELATIONSHIP TO THE FORMATION OF ENTERIC GANGLIA
复制标题
DOI：
10.1002/cne.903130408
复制
发表时间：
1991-11-22
影响因子：
2.5
通讯作者：
GERSHON, MD
中科院分区：
文献类型：
--
作者：
POMERANZ, HD;SHERMAN, DL;GERSHON, MD
为了产生肠神经系统（ENS），从神经嵴迁移的细胞必须找到肠道并在肠道内的适当位置停止迁移。以前的研究的ENS在突变小鼠的发展导致的假设，层粘连蛋白在肠间充质中可能作为一个信号，嵴源性细胞停止迁移和延长神经突（或神经胶质过程）。在这一假设中暗示的是这样的想法，即嵴源性细胞，作为它们参与神经节形成的前奏，获得神经相关的层粘连蛋白受体，它们在肠前阶段的迁移中不表达。作为这一假设的部分测试，单和双标记免疫细胞化学在光和电子显微镜（EM）水平被用来研究细胞表面层粘连蛋白结合蛋白的表达的波峰衍生的细胞在迁移过程中或在发展中的鸡肠道内。两种针对神经元细胞表面层粘连蛋白结合蛋白产生的抗体（称为3070和α-110）用于此目的。层粘连蛋白结合蛋白的免疫反应性被发现表达在肠和神经节的Remak由一个子集的嵴源性细胞（确定免疫细胞化学与NC-1/HNK-1抗体）和所有的发展为神经元（确定免疫细胞化学与抗体的神经元相关蛋白）。层粘连蛋白结合蛋白免疫反应性也被发现表达在固定的神经结构在胚胎的其他地方，包括颅和脊神经根，神经和神经节。相比之下，层粘连蛋白结合蛋白免疫反应性不表达于神经轴迷走神经或骶骨区附近的迁移嵴衍生细胞（ENS的前体起源于此）;也不表达于近咽迷走嵴衍生细胞通过尾鳃弓迁移到前肠或通过接近后肠的骶骨嵴衍生细胞的尾流。EM免疫细胞化学证实，层粘连蛋白结合蛋白的免疫反应性在肠位于嵴源性细胞的表面上，并表现出这些细胞，只能从他们的邻居区分他们的NC-1/HNK-1的免疫反应性和细胞发展为神经元或胶质细胞。EM免疫细胞化学还显示，通过肠间充质迁移的嵴源性细胞的表面接触许多小的嗜锇性层粘连蛋白免疫反应性细胞外物质的“泡芙”。这些泡芙与表达层粘连蛋白结合蛋白的免疫反应性的膜位点的位置相吻合。这些观察结果与层粘连蛋白在肠神经节形成中起作用的假设一致。
In order to give rise to the enteric nervous system (ENS), cells migrating from the neural crest must find the bowel and cease migrating at appropriate locations within the gut. Previous studies of the development of the ENS in a mutant mouse have led to the hypothesis that laminin in the enteric mesenchyme may act as a signal to crest-derived cells to cease migrating and extend neurites (or glial processes). Implied in this hypothesis is the idea that crest-derived cells, as a prelude to their participation in ganglion formation, acquire a neurally related laminin receptor, which they do not express at pre-enteric stages of migration. As a partial test of this hypothesis, single and double label immunocytochemistry at light and electron microscopic (EM) levels were used to study the expression of cell surface laminin binding proteins by crest-derived cells in the process of migrating to or within the developing chick gut. Two antibodies (called 3070 and alpha-110) raised against neuronal cell surface laminin binding proteins were employed for this purpose. Laminin binding protein immunoreactivity was found to be expressed within the bowel and ganglion of Remak by a subset of crest-derived cells (identified immunocytochemically with NC-1/HNK-1 antibodies) and by all of those developing as neurons (identified immunocytochemically with antibodies to neurofilament-associated proteins). Laminin binding protein immunoreactivity was also found to be expressed in fixed neural structures elsewhere in the embryos, including cranial and spinal roots, nerves, and ganglia. In contrast, laminin binding protein immunoreactivity was not expressed by migrating crest-derived cells in the vicinity of the vagal or sacral regions of the neuraxis (from which the precursors of the ENS take origin); nor was it expressed by juxta-pharyngeal vagal crest-derived cells migrating to the foregut through the caudal branchial arches or by the caudal stream of sacral crest-derived cells approaching the hindgut. EM immunocytochemistry confirmed that laminin binding protein immunoreactivity in the bowel was located on the surfaces of crest-derived cells, and was exhibited both by those cells that could only be distinguished from their neighbors by their NC-1/HNK-1 immunoreactivity and by cells developing as neurons or glia. EM immunocytochemistry also revealed that the surfaces of crest-derived cells migrating through the enteric mesenchyme were contacted by many small osmiophilic "puffs" of laminin-immunoreactive extracellular material. These puffs coincided in location with membrane sites that expressed the immunoreactivity of the laminin binding protein. These observations are consistent with the hypothesis that laminin plays a role in the formation of enteric ganglia.