BASIC FIBROBLAST GROWTH-FACTOR IMPROVES MYOCARDIAL-FUNCTION IN CHRONICALLY ISCHEMIC PORCINE HEARTS
BASIC FIBROBLAST GROWTH-FACTOR IMPROVES MYOCARDIAL-FUNCTION IN CHRONICALLY ISCHEMIC PORCINE HEARTS
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DOI：
10.1172/jci117378
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发表时间：
1994-08-01
影响因子：
15.9
通讯作者：
SIMONS, M
中科院分区：
文献类型：
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作者：
HARADA, K;GROSSMAN, W;SIMONS, M
在26只猪冠状动脉近端回旋支(Lcx)节段性缩窄器上，观察了碱性成纤维细胞生长因子(BFGF)对慢性缺血心脏局部心肌功能和血流的影响。在13只动物中，在原始左前降支(LAD)和左冠状动脉左前降支(LAD)和左冠状动脉前降支(LCX)动脉注射碱性成纤维细胞生长因子(BFGF)，并用肝素-海藻酸盐珠和其他13只动物作为对照。经碱性成纤维细胞生长因子治疗的猪显示，与未经治疗的对照组相比，左心室梗死面积缩小了四倍(梗死面积：左心室重量的1.2+/-0.4%对5.1+/-1.3%，平均+/-扫描电子显微镜，P<；0.05)。与LAD区相比，bFGF组和对照组的静息状态下Lcx区短轴缩短率(%FS)均降低。然而，在快速起搏后，bFGF组的Lcx区的恢复(%FSLCX/%FSLAD+/-22.9+/-7.3%，30.5+/-8.5%，P<；0.05比起搏前(16.0+/-7.8%-->；143+/-7.0%，P=NS)要好于对照组。此外，快速起搏时bFGF组的LV舒张末压力升高小于对照组(起搏前、起搏后、起搏后，10+/-1；17+/-3；11+/-1*mm Hgvs 10+/-1；24+/-4；15+/-1 mm Hg，*P<；0.05与对照组相比)。在使用碱性成纤维细胞生长因子的猪的起搏过程中，Lcx区域的冠脉血流量(LAD血流量归一化)也比对照组好。因此，在猪的渐进性冠状动脉闭塞模型中，外周应用bFGF可以改善冠状动脉血流，缩小受损区域的梗塞面积，并防止起搏引起的血流动力学恶化。
The effect of basic fibroblast growth factor (bFGF) administration on regional myocardial function and blood flow in chronically ischemic hearts was studied in 26 pigs instrumented with proximal circumflex coronary artery (LCX) ameroid constrictors. In 13 animals bFGF was administered extraluminally to the primal left anterior descending (LAD) and LCX arteries with heparin-alginate beads and 13 other animal served as controls. bFGF-treated pigs showed a fourfold reduction in left ventricular infarct size compared to untreated controls (infarct size: 1.2 +/- 0.4% vs. 5.1 +/- 1.3% of LV mass, mean +/- SEM, P < 0.05). Percent fractional shortening (% FS) in the LCX area at rest was reduced compared with the LAD region in both bFGF and control pigs. However, there was better recovery in the LCX area after rapid pacing in bFGF-treated pigs (% FSLCX/% FSLAD +/- 22.9 +/- 7.3% --> 30.5 +/- 8.5%, P < 0.05 vs. prepacing) than in controls (16.0 +/- 7.8% --> 143 +/- 7.0%, P = NS). Furthermore, LV end-diastolic pressure rise with rapid pacing was less in bFGF-treated than control pigs (pre-pacing; pacing; postpacing, 10 +/- 1; 17 +/- 3; 11 +/- 1* mmHg vs 10 +/- 1; 24 +/- 4; 15 +/- 1 mmHg, *P < 0.05 vs. control). Coronary blood flow in the LCX territory (normalized for LAD flow) was also better during pacing in bFGF-treated pigs than in controls. Thus, periadventitial administration of bFGF in a gradual coronary occlusion model in pigs results in improvement of coronary flow and reduction in infarct size in the compromised territory as well as in prevention of pacing-induced hemodynamic deterioration.