Chimeric peptide constructs comprising linear B-cell epitopes: application to the serodiagnosis of infectious diseases.
Chimeric peptide constructs comprising linear B-cell epitopes: application to the serodiagnosis of infectious diseases.
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DOI：
10.1038/srep13364
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发表时间：
2015-08-21
影响因子：
4.6
通讯作者：
Ma H
中科院分区：
文献类型：
--
作者：
Lu Y;Li Z;Teng H;Xu H;Qi S;He J;Gu D;Chen Q;Ma H
线性B细胞表位是感染性疾病血清学诊断的理想生物标志物。然而，长期预测的表位诊断价值尚未实现。在这里，我们展示了一种方法，四步诊断表位（DEIFS），它提供了一个高成功率的传染病血清诊断表位的组合。使用疟疾的DEIFS，我们从8个肽中鉴定出6个表位，并将它们组合成3个嵌合肽构建体。沿着4种其他肽，我们开发了一种快速诊断测试（RDT），其能够区分恶性疟原虫（P. falciparum）与间日疟原虫（P. vivax）感染，具有95.6%的总体灵敏度和99.1%的总体特异性。除了在诊断中的应用外，DEIFS还可用于病毒和细菌感染的诊断、候选疫苗的发现、疫苗效力的评估以及疾病进展的研究。
Linear B-cell epitopes are ideal biomarkers for the serodiagnosis of infectious diseases. However, the long-predicted diagnostic value of epitopes has not been realized. Here, we demonstrated a method, diagnostic epitopes in four steps (DEIFS), that delivers a combination of epitopes for the serodiagnosis of infectious diseases with a high success rate. Using DEIFS for malaria, we identified 6 epitopes from 8 peptides and combined them into 3 chimeric peptide constructs. Along with 4 other peptides, we developed a rapid diagnostic test (RDT), which is able to differentiate Plasmodium falciparum (P. falciparum) from Plasmodium vivax (P. vivax) infections with 95.6% overall sensitivity and 99.1% overall specificity. In addition to applications in diagnosis, DEIFS could also be used in the diagnosis of virus and bacterium infections, discovery of vaccine candidates, evaluation of vaccine potency, and study of disease progression.