P1B15 - A CDNA CLONE OF THE RAT MESSENGER-RNA ENCODING CYCLOPHILIN
P1B15 - A CDNA CLONE OF THE RAT MESSENGER-RNA ENCODING CYCLOPHILIN
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DOI：
10.1089/dna.1988.7.261
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发表时间：
1988-05-01
期刊：
影响因子：
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通讯作者：
SUTCLIFFE, JG
中科院分区：
文献类型：
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作者：
DANIELSON, PE;FORSSPETTER, S;SUTCLIFFE, JG
我们展示了编码大鼠亲环蛋白的 cDNA 的完整核苷酸序列。 743个核苷酸的序列包含42个核苷酸的5''非编码区、对应于分子量为17,874的164个氨基酸的翻译产物的492个核苷酸的开放阅读框和209个核苷酸的3''非编码区。引物延伸研究揭示了一个次要转录起始位点和两个主要转录起始位点的存在。 Southern 印迹分析与多达 20 个亲环蛋白基因拷贝和可能的假基因一致。亲环蛋白 mRNA 在大鼠和猴的几乎所有类型的组织中表达，并且在哺乳动物进化过程中似乎高度保守。
We present the complete nucleotide sequence of a cDNA encoding rat cyclophilin. The 743-nucleotide sequence contains a 42-nucleotide 5'' noncoding region, a 492 nucleotide open reading frame corresponding to a translation product of 164 amino acids with a molecular weight of 17,874, and a 3'' noncoding region of 209 nucleotides. Primer extension studies reveal the presence of a one minor and two major transcription start sites. Southern blot analyses are consistent with as many as 20 copies of the cylophilin gene and possible pseudogenes. Cyclophilin mRNA is expressed in virtually all types of tissues of rat and monkey and appears to have been highly conserved during mammalian evolution.