Nitric Oxide Synthase Inhibitor Does Not Reduce Minimum Alveolar Anesthetic Concentration of Halothane in Rats
Nitric Oxide Synthase Inhibitor Does Not Reduce Minimum Alveolar Anesthetic Concentration of Halothane in Rats
复制标题
一氧化氮合酶抑制剂不会降低大鼠氟烷的最低肺泡麻醉浓度
作者：
T. Adachi;J. Kurata;S. Nakao;M. Murakawa;T. Shichino;G. Shirakami;T. Shinomura;K. Mori
据报道，一氧化氮（NO）合酶抑制剂（Nω-硝基-L-精氨酸甲酯[L-NAME]）在静脉（IV）给药时可降低氟烷的最低肺泡麻醉浓度（MAC），在脑室内（ICV）或鞘内（IT）给药时可降低热痛觉过敏或在福尔马林试验中产生抗伤害感受。本研究试图确定L-NAME在中枢神经系统（CNS）中降低氟烷MAC的作用部位。为此，我们研究了静脉，ICV，和IT管理的L-NAME氟烷MAC大鼠的影响。与早期的研究相反，我们没有观察到静脉注射（10-30 mg/kg）L-NAME后氟烷MAC的任何降低。ICV（100 pg）和IT（100 pg和1 mg）给予L-NAME也没有改变氟烷MAC。这些结果表明，L-精氨酸-NO通路不参与氟烷抑制机械伤害性反应的作用机制或机械刺激的伤害性神经机制。
Nitric oxide (NO) synthase inhibitor (Nω-nitro-L-arginine methyl ester [L-NAME]) has been reported to reduce minimum alveolar anesthetic concentration (MAC) of halothane when administered intravenously (IV) and to reduce thermal hyperalgesia, or produce antinociception in the formalin test, when administered intracerebroventricularly (ICV) or intrathecally (IT). This study attempts to identify the site(s) in the central nervous system (CNS) where L-NAME acts to reduce the halothane MAC. For this purpose, we examined the effects of IV, ICV, and IT administration of L-NAME on the halothane MAC in rats. In contrast to an earlier study, we did not observe any decrease in the halothane MAC after IV (10–30 mg/kg) administration of L-NAME. ICV (100 pg) and IT (100 pg and 1 mg) administration of L-NAME also did not alter the halothane MAC. These findings indicate that the L-arginine-NO pathway is not involved in the mechanism of action of halothane to suppress mechanical nociceptive response or in the nociceptive neural mechanism of mechanical stimulation.