Comparative SEM and TEM observations of nanoleakage within the hybrid layer.
Comparative SEM and TEM observations of nanoleakage within the hybrid layer.
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DOI：
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发表时间：
1995
影响因子：
2.2
通讯作者：
H. Sano;M. Yoshiyama;S. Ebisu;M. Burrow;T. Takatsu;B. Ciucchi;R. Carvalho;D. Pashley
中科院分区：
文献类型：
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作者：
H. Sano;M. Yoshiyama;S. Ebisu;M. Burrow;T. Takatsu;B. Ciucchi;R. Carvalho;D. Pashley
大多数粘合剂界面研究涉及粘合剂树脂渗透到脱矿牙本质表面的SEM演示，随后创建混合层。纳米泄漏是描述在没有间隙形成的情况下小离子或分子在混合层内扩散的术语。本显微镜研究使用银染色技术检查混合层的纳米渗漏。使用Clearfil Liner Bond和All-Bond 2粘合剂系统制备浸入硝酸银溶液中的粘合剂牙本质三明治，用于SEM和TEM检查。两种系统均显示混合层内存在银蓄积。通过SEM和TEM观察，Clearfil Liner Bond System在混合层的底部三分之二处显示分散的银颗粒，而All-Bond 2在混合层的整个厚度内显示染色的纤维状结构。为了评估混合层的质量，提出了利用可通过SEM和TEM两者检测的示踪剂分子如硝酸银。重要的是要确定这些纳米尺寸的孔隙的位置和形态，可能允许胶原纤维的水解和粘合剂单体的降解。
Most adhesive interface studies have involved SEM demonstration of the penetration of adhesive resins into demineralized dentin surfaces with subsequent creation of hybrid layers. Nanoleakage is a term that describes the diffusion of small ions or molecules within the hybrid layer in the absence of gap formation. The present microscopic study examined the nanoleakage of the hybrid layer using a silver nitrate staining technique. Adhesive dentin sandwiches, which were immersed in a silver nitrate solution, were prepared for both SEM and TEM examination using both the Clearfil Liner Bond and All-Bond 2 adhesive systems. Both systems demonstrated silver accumulation within the hybrid layers. Clearfil Liner Bond System showed scattered silver particles at the bottom two-thirds of the hybrid layer by both SEM and TEM observation, whereas All-Bond 2 revealed stained fiber-like structures within the full thickness of the hybrid layer. To evaluate the quality of the hybrid layer, the utilization of tracer molecules such as silver nitrate that are detectable by both SEM and TEM is proposed. It is important to determine the location and morphology of these nanometer-sized porosities that may permit the hydrolysis of collagen fibers and degradation of adhesive monomers.