Lactobacillus casei strain Shirota suppresses serum immunoglobulin E and immunoglobulin G1 responses and systemic anaphylaxis in a food allergy model
Lactobacillus casei strain Shirota suppresses serum immunoglobulin E and immunoglobulin G1 responses and systemic anaphylaxis in a food allergy model
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DOI：
10.1046/j.0954-7894.2002.01354.x
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发表时间：
2002-04-01
影响因子：
6.1
通讯作者：
Kaminogawa, S
中科院分区：
文献类型：
--
作者：
Shida, K;Takahashi, R;Kaminogawa, S
背景我们先前使用变应原致敏的小鼠脾细胞培养物的研究表明，干酪乳杆菌菌株Shirota（LcS），一种广泛用作发酵乳制品发酵剂的乳酸菌，通过促进由IL-1介导的显性Th 1型应答抑制IgE产生，目的我们试图使用食物过敏模型评估US在体内抑制IgE应答和过敏反应的能力，方法检测热灭活的LcS诱导小鼠血清中IL-12的能力。OVA-TCR-Tg小鼠喂食含有OVA的饮食4周，并在此期间的第一周腹膜内注射LcS三次。检查脾细胞的细胞因子和抗体分泌以及血清IgE和IgG 1应答。结果腹腔注射LcS可诱导OVA-TCR-Tg小鼠血清产生IL-12反应。在食物过敏模型中，LcS给药使脾细胞产生细胞因子的模式向Th 1优势倾斜，并抑制脾细胞分泌IgE和IgG 1。LcS调节细胞因子产生的能力被抗IL-12抗体处理阻断。LcS还抑制血清OVA特异性IgE和IgG 1反应，减少全身过敏性反应。结论美国管理抑制IgE和IgG 1反应和全身过敏反应的食物过敏模型，这表明可能使用这种乳酸菌在预防食物过敏。
Background Our previous study using allergen-sensitized murine splenocyte cultures has shown that Lactobacillus casei strain Shirota (LcS), a lactic acid bacterium widely used as a starter for fermented milk products, suppresses IgE production through promoting a dominant Th1-type response mediated by IL-12 induction.Objective We tried to evaluate the ability of US to suppress both IgE response and allergic reactions in vivo using a food allergy model with ovalbumin-specific T cell receptor transgenic (OVA-TCR-Tg) mice.Methods The ability of heat-killed LcS to induce IL-12 in serum was tested. OVA-TCR-Tg mice were fed a diet containing OVA for 4 weeks and injected with LcS intraperitoneally three times in the first week of this period. Cytokine and antibody secretion by splenocytes, and serum IgE and IgG1 responses were examined. The inhibitory effect of LcS on systemic anaphylaxis induced by intravenous challenge of OVA-fed OVA-TCR-Tg mice with OVA was also tested.Results Intraperitoneal injection of LcS induced an IL-12 response in the serum of OVA-TCR-Tg mice. In the food allergy model, LcS administration skewed the pattern of cytokine production by splenocytes toward Th1 dominance, and suppressed IgE and IgG1 secretion by splenocytes. The ability of LcS to modulate cytokine production was blocked by anti-IL-12 antibody treatment. LcS also inhibited serum OVA-specific IgE and IgG1 responses and diminished systemic anaphylaxis.Conclusion US administration suppresses IgE and IgG1 responses and systemic allergic reactions in a food allergy model, suggesting a possible use of this lactic acid bacterium in preventing food allergy.