CODON USAGE IN BACTERIA - CORRELATION WITH GENE EXPRESSIVITY
CODON USAGE IN BACTERIA - CORRELATION WITH GENE EXPRESSIVITY
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DOI：
10.1093/nar/10.22.7055
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
GAUTIER, C
中科院分区：
文献类型：
--
作者：
GOUY, M;GAUTIER, C
核酸序列条带现在包含超过600个蛋白质编码基因，其中107个来自原核生物。每个新的原核基因的密码子频率。在大肠杆菌基因组（染色体，转座子和质粒）的83个测序基因的遗传密码使用的分析，考虑到新的数据，基因表达和调节，以及异tRNA特异性和细胞浓度。每个基因的密码子组成用两个指标来概括：一个是基于异tRNA种类在基因翻译过程中的差异使用，另一个是基于胞嘧啶和尿嘧啶作为第三碱基的选择。密码子组成和mRNA表达率之间的强关系得到证实，即使是在同一操纵子中转录的基因。讨论了密码子的使用对多肽延伸率和蛋白质产量的影响。最后，进化方面的密码子选择mRNA序列进行了研究。
The nucleic acid sequence band now contains over 600 protein coding genes of which 107 are from prokaryotic organisms. Codon frequencies in each new prokaryotic gene are given. Analysis of genetic code usage in the 83 sequenced genes of the Escherichia coli genome (chromosome, transposons and plasmids) is presented, taking into account new data on gene expressivity and regulation as well as iso-tRNA specificity and cellular concentration. The codon composition of each gene is summarized using two indexes: one is based on the diferential usage of iso-tRNA species during gene translation, the other on choice between Cytosine and Uracile for third base. A strong relationship between codon composition and mRNA expressivity is confirmed, even for genes transcribed in the same operon. The influence of codon use on peptide elongation rate and protein yield is discussed. Finally, the evolutionary aspect of codon selection in mRNA sequences is studied.